Literature summary extracted from

Li, J.Y.; Fu, Y.L.; Pike, S.M.; Bao, J.; Tian, W.; Zhang, Y.; Chen, C.Z.; Zhang, Y.; Li, H.M.; Huang, J.; Li, L.G.; Schroeder, J.I.; Gassmann, W.; Gong, J.M.
The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance (2010), Plant Cell, 22, 1633-1646.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
7.3.2.4	additional information	the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype, and nitrate transport by NRT1.8 is pH-dependent	Arabidopsis thaliana

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
7.3.2.4	ansient expression of the NRT1.8:enhanced green fluorescent protein fusion in onion epidermal cells and Arabidopsis protoplasts	Arabidopsis thaliana

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
7.3.2.4	Cd2+	the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype	Arabidopsis thaliana

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
7.3.2.4	plasma membrane	subcellular localization study, overview	Arabidopsis thaliana	5886	-

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
7.3.2.4	Cd2+	the nrt1.8-1 mutant shows a nitrate-dependent Cd2+-sensitive phenotype, while wild-type NRT1.8 is upregulated by Cd2+ stress	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
7.3.2.4	ATP + H2O + nitrate/out	Arabidopsis thaliana	NRT1.8 can transport nitrate and regulates nitrate removal from xylem. Nitrate distribution under Cd2+ stress is altered by NRT1.8	ADP + phosphate + nitrate/in	-	?
7.3.2.4	additional information	Arabidopsis thaliana	NRT1.8, injected in Xenopus laevis oocytes, mediates low-affinity nitrate uptake	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
7.3.2.4	Arabidopsis thaliana	-	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
7.3.2.4	leaf	-	Arabidopsis thaliana	-
7.3.2.4	parenchyma	histochemical analysis shows that NRT1.8 is expressed predominantly in xylem parenchyma cells within the vasculature	Arabidopsis thaliana	-
7.3.2.4	xylem	histochemical analysis shows that NRT1.8 is expressed predominantly in xylem parenchyma cells within the vasculature	Arabidopsis thaliana	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
7.3.2.4	ATP + H2O + nitrate/out	-	Arabidopsis thaliana	ADP + phosphate + nitrate/in	-	?
7.3.2.4	ATP + H2O + nitrate/out	NRT1.8 can transport nitrate and regulates nitrate removal from xylem. Nitrate distribution under Cd2+ stress is altered by NRT1.8	Arabidopsis thaliana	ADP + phosphate + nitrate/in	-	?
7.3.2.4	additional information	NRT1.8, injected in Xenopus laevis oocytes, mediates low-affinity nitrate uptake	Arabidopsis thaliana	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
7.3.2.4	More	NRT1.8 is a member of the nitrate transporter, NRT1, family	Arabidopsis thaliana
7.3.2.4	nitrate transporter	-	Arabidopsis thaliana
7.3.2.4	NRT1.8	-	Arabidopsis thaliana

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3.2.4	additional information	-	nitrate transport by NRT1.8 is pH-dependent	Arabidopsis thaliana

Expression

EC Number	Organism	Comment	Expression
7.3.2.4	Arabidopsis thaliana	NRT1.8 is upregulated by nitrate. NRT1.8 is the only nitrate assimilatory pathway gene that is strongly upregulated by Cd2+ stress in roots	up

General Information

EC Number	General Information	Comment	Organism
7.3.2.4	metabolism	NRT1.8 is the only nitrate assimilatory pathway gene that is strongly upregulated by Cd2+ stress in roots. NRT1.8-regulated nitrate distribution plays an important role in Cd2+ tolerance	Arabidopsis thaliana
7.3.2.4	additional information	functional disruption of NRT1.8 significantly increased the nitrate concentration in xylem sap	Arabidopsis thaliana
7.3.2.4	physiological function	nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance	Arabidopsis thaliana

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Release 2023.1 (January 2023)