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Literature summary extracted from

  • Nagaraj, V.A.; Prasad, D.; Arumugam, R.; Rangarajan, P.N.; Padmanaban, G.
    Characterization of coproporphyrinogen III oxidase in Plasmodium falciparum cytosol (2010), Parasitol. Int., 59, 121-127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.3.3 functional expression in Escherichia coli Plasmodium falciparum
1.3.3.3 recombinant expression of His-tagged enzyme in Escherichia coli Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.3.3 0.00967
-
 Coproporphyrinogen III pH 7.5, 37°C, recombinant enzyme Plasmodium falciparum
1.3.3.3 0.0151
-
 Coproporphyrinogen III pH 7.5, 37°C, native enzyme Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.3.3 cytosol
-
 Plasmodium falciparum 5829
-
1.3.3.3 cytosol exclusively Plasmodium falciparum 5829
-
1.3.3.3 additional information no localization in the mitochondrion Plasmodium falciparum
-
-
1.3.3.3 additional information PfCPO does not carry an obvious N-terminal leader targeting sequence, subcellular localization study, overview. No measurable activity in the solubilized parasite membrane fraction Plasmodium falciparum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.3.3 additional information metal ions Cu2+, Mg2+, Mn2+, Fe2+ and Zn2+ at up to 0.500 mM concentration have no effect on CPO activity Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.3.3 58000
-
 1 * 58000, recombinant enzyme, SDS-PAGE Plasmodium falciparum
1.3.3.3 61000
-
 recombinant enzyme, gel filtration Plasmodium falciparum
1.3.3.3 61000
-
 recombiinant enzyme, gel filtration Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.3 coproporphyrinogen III + O2 + 2 H+ Plasmodium falciparum
-
 protoporphyrinogen IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen III + O2 + 2 H+ Plasmodium falciparum
-
 protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.3 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.3.3 recombinant His-tagged enzyme from Escherichia coli, cleavage of the tag Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.3 coproporphyrinogen III + O2 + 2 H+
-
 Plasmodium falciparum protoporphyrinogen IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 coproporphyrinogen III + O2 + 2 H+
-
 Plasmodium falciparum protoporphyrinogen-IX + 2 CO2 + 2 H2O
-
 ?
1.3.3.3 additional information no activity with S-adenosylmethionine or without O2 Plasmodium falciparum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.3.3 monomer 1 * 58000, recombinant enzyme, SDS-PAGE Plasmodium falciparum
1.3.3.3 More sequence comparisons, overview Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
1.3.3.3 coproporphyrinogen III oxidase
-
 Plasmodium falciparum
1.3.3.3 CPO
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.3.3 37
-
-
 Plasmodium falciparum
1.3.3.3 37
-
 recombinant enzyme Plasmodium falciparum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.3.3 25 50 activity range, recombinant enzyme Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.3.3 7.5
-
-
 Plasmodium falciparum
1.3.3.3 7.6
-
 recombinant enzyme Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.3.3 6 9 activity range, recombinant enzyme Plasmodium falciparum