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Literature summary extracted from

  • Ko, H.J.; Lee, E.W.; Bang, W.G.; Lee, C.K.; Kim, K.H.; Choi, I.G.
    Molecular characterization of a novel bacterial aryl acylamidase belonging to the amidase signature enzyme family (2010), Mol. Cells, 29, 485-492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.13 DNA and amino acid sequence determination and analysis, phylogenetic analysis, genetic library construction and cloning in Escherichia coli strains NM522 and DH5alpha, expression in Escherichia coli strain BL21(DE3) bacterium CSBL00001

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.13 Ca2+ inhibits 21.1% at 1 mM and 21.5% at 10 mM bacterium CSBL00001
3.5.1.13 Co2+ inhibits 14.1% at 1 mM and 17.4% at 10 mM bacterium CSBL00001
3.5.1.13 Cu2+ inhibits 13.4% at 1 mM and 45.7% at 10 mM bacterium CSBL00001
3.5.1.13 Ni2+ inhibits 26.0% at 1 mM and 43.2% at 10 mM bacterium CSBL00001
3.5.1.13 Zn2+ inhibits 40.0% at 1 mM and 75.8% at 10 mM bacterium CSBL00001

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.13 0.1
-
 4-nitroacetanilide pH 9.0, 37°C bacterium CSBL00001
3.5.1.13 0.32
-
 N-(4-hydroxyphenyl)acetamide pH 9.0, 37°C bacterium CSBL00001
3.5.1.13 0.83
-
 4-chloroacetanilide pH 9.0, 37°C bacterium CSBL00001
3.5.1.13 19
-
 acetanilide pH 9.0, 37°C bacterium CSBL00001

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.13 4-acetamidophenol + H2O bacterium CSBL00001 i.e. Tylenol acetate + 4-aminophenol
-
 r
3.5.1.13 4-chloroacetanilide + H2O bacterium CSBL00001
-
 acetate + 4-chloroaniline
-
 r
3.5.1.13 4-nitroacetanilide + H2O bacterium CSBL00001
-
 acetate + 4-nitroaniline
-
 r
3.5.1.13 acetanilide + H2O bacterium CSBL00001
-
 acetate + aniline
-
 r
3.5.1.13 phenacetin + H2O bacterium CSBL00001
-
 acetate + 4-ethoxyaniline
-
 r

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.13 bacterium CSBL00001 C3UWD1 a soil bacterium, might be a Pseudomonas species
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.13 4-acetamidophenol + H2O i.e. Tylenol bacterium CSBL00001 acetate + 4-aminophenol
-
 r
3.5.1.13 4-acetamidophenol + H2O i.e. Tylenol. The enzyme contains a revealed a conserved amidase signature region with a canonical catalytic triad bacterium CSBL00001 acetate + 4-aminophenol
-
 r
3.5.1.13 4-chloroacetanilide + H2O
-
 bacterium CSBL00001 acetate + 4-chloroaniline
-
 r
3.5.1.13 4-nitroacetanilide + H2O
-
 bacterium CSBL00001 acetate + 4-nitroaniline
-
 r
3.5.1.13 acetanilide + H2O
-
 bacterium CSBL00001 acetate + aniline
-
 r
3.5.1.13 phenacetin + H2O
-
 bacterium CSBL00001 acetate + 4-ethoxyaniline
-
 r
3.5.1.13 phenacetin + H2O i.e. N-(4-ethoxyphenyl)acetamide bacterium CSBL00001 acetate + 4-ethoxyaniline
-
 r

Synonyms

EC Number Synonyms Comment Organism
3.5.1.13 AAA
-
 bacterium CSBL00001
3.5.1.13 More the enzyme belongs to the amidase signature, AS, enzyme family bacterium CSBL00001

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.13 37
-
 recombinant enzyme bacterium CSBL00001

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.13 37 40 purified recombinant enzyme, pH 9.0, the half-life of enzyme activity at 37°C is 192 h, and 90% of its activity remain after 3 h incubation at 40°C bacterium CSBL00001
3.5.1.13 45
-
 purified recombinant enzyme, 3 h, pH 9.0, stable up to bacterium CSBL00001
3.5.1.13 55
-
 purified recombinant enzyme, 3 h, pH 9.0, 50% activity remaining bacterium CSBL00001
3.5.1.13 70
-
 purified recombinant enzyme, 3 h, pH 9.0, inactivation bacterium CSBL00001

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.13 5 7 reverse reaction bacterium CSBL00001
3.5.1.13 10
-
 recombinant enzyme, forward reaction bacterium CSBL00001