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Literature summary extracted from

  • Gang, J.G.; Yun, S.K.; Hwang, S.Y.
    Helicobacter pylori urease may exist in two forms: evidence from the kinetic studies (2009), J. Microbiol. Biotechnol., 19, 1565-1568.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.5 glycerol optimum concentration: 15% Helicobacter pylori
3.5.1.5 n-octylglucoside optimum concentration: 0.1% Helicobacter pylori
3.5.1.5 PEG optimum concentration: 0.5% Helicobacter pylori
3.5.1.5 SDS optimum concentration: 0.02% Helicobacter pylori
3.5.1.5 Triton X-100 optimum concentration: 0.05% Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.5 2-mercaptoethanol
-
 Helicobacter pylori
3.5.1.5 mercaptoethylamine inactivates enzyme irreversibly Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.5 additional information
-
 additional information sigmoid curve at low urea concentration (below 0.1 mM). In the presence of glycerol or PEG (preservatives), the sigmoid pattern changes to a rectangular hyperbola, and urea hydrolysis is consistent with Michaelis-Menten kinetics. Based on the kinetics of urease in the presence of the preservatives, the two forms of urease i.e., (alpha beta)3 and (alpha beta)6 may well exist in Helicobacter pylori. The hyperbolic kinetics adopted by the dissociated form of urease may allow for maximal urea assimilation by Helicobacter pylori under acidic conditions and at low urea concentration Helicobacter pylori
3.5.1.5 0.21
-
 Urea pH 6.8, 37°C, Vmax: 1200 micromol/min/mg, in the presence of preservatives PEG or glycerol Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.5 29000
-
 SDS-PAGE, subunits of the purified urease are shown to be stoichiometrically equal, with estimated molecular masses of 63 kDa and 29kDa Helicobacter pylori
3.5.1.5 63000
-
 SDS-PAGE, subunits of the purified urease are shown to be stoichiometrically equal, with estimated molecular masses of 63 kDa and 29kDa Helicobacter pylori
3.5.1.5 580000
-
 gel filtration Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.5 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.5 using NH4(SO4)2 precipitation, phenyl-Sepharose column chromatography and gel filtration Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.5 urea + H2O
-
 Helicobacter pylori CO2 + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.5 hexamer purified enzyme may be a hexameric aggregate, consistent with the reported stoichiometry of (29-63 kDa) * 6 of Helicobacter pylori Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
3.5.1.5 urease
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.5 37
-
 assay at Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.5 6.8
-
 assay at Helicobacter pylori

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.5 4
-
 enzyme gets irreversibly inactivated at pH 4 Helicobacter pylori