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Literature summary extracted from

  • Krzeslak, J.; Braun, P.; Voulhoux, R.; Cool, R.H.; Quax, W.J.
    Heterologous production of Escherichia coli penicillin G acylase in Pseudomonas aeruginosa (2009), J. Biotechnol., 142, 250-258.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.11 expression of functional wild-type and mutant enzymes in Pseudomonas aeruginosa PAO1 and PAO1DELTAtatABC strains using the pMMB67EH shuttle vector, the mutant a TatProPGA hybrid only shows an active site, when the transformed bacterium is grown at 25°C or lower temperatures. Processing of recombinant PGA can also occur in the cytoplasm of Pseudomonas aeruginosa. The extracellular localization of the TatProPGA hybrid is not dependent on the tatABC-genes Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.11 additional information construction of a fusion protein, consisting of Pseudomonas Sec- or Tat-specific signal peptides, the elastase propeptide and the mature penicillin G acylase, termed a TatProPGA hybrid. the mature protein, expressed from a TatProPGA hybrid, is not only found in the extracellular medium and the periplasm, but also in the cytoplasm as assessed by comparison to the reporter beta-lactamase protein Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.11 cytosol the mature protein, expressed from a TatProPGA hybrid, is not only found in the extracellular medium and the periplasm, but also in the cytoplasm as assessed by comparison to the reporter beta-lactamase protein Escherichia coli 5829
-
3.5.1.11 extracellular the mature protein, expressed from a TatProPGA hybrid, is not only found in the extracellular medium and the periplasm, but also in the cytoplasm as assessed by comparison to the reporter beta-lactamase protein Escherichia coli
-
-
3.5.1.11 periplasm the mature protein, expressed from a TatProPGA hybrid, is not only found in the extracellular medium and the periplasm, but also in the cytoplasm as assessed by comparison to the reporter beta-lactamase protein Escherichia coli
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.11 Escherichia coli
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.1.11 proteolytic modification the signal peptide is removed from the ppPGA during translocation into the periplasm. In the periplasm pPGA undergoes posttranslational modification that results in active PGA. Processing of the PGA is temperature-dependent Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.11 6-nitro-3-phenylacetamide benzoic acid + H2O
-
 Escherichia coli 3-amino-6-nitrobenzoic acid + phenylacetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.11 penicillin G acylase
-
 Escherichia coli
3.5.1.11 PGA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.11 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.11 7
-
 assay at Escherichia coli