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Literature summary extracted from

  • Mao, Z.; Sun, W.; Xu, R.; Novgorodov, S.; Szulc, Z.M.; Bielawski, J.; Obeid, L.M.; Mao, C.
    Alkaline ceramidase 2 (ACER2) and its product dihydrosphingosine mediate the cytotoxicity of 4-HPR in tumor cells (2010), J. Biol. Chem., 285, 29087-29090.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.23 transient expression of FLAG-tagged ACER3 in HSC-1 cells. ACER2 overexpression in HeLa cells, knockdown by RNA interference with ACER2-specific siRNA Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.23 additional information overexpression of ACER2 augments the 4-N-(4-hydroxyphenyl)retinamide-induced generation of dihydrosphingosine, as well as 4-N-(4-hydroxyphenyl)retinamide cytotoxicity and death in tumor cells, whereas knocking down ACER2 had the opposite effects. ACER2 overexpression or GT11, a dihydrosphingosine desaturase inhibitor, treatment alone caused little or no decrease in cell viability, whereas ACER2 overexpression along with GT11 treatment markedly decreased cell viability Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 Golgi apparatus ACER2 Homo sapiens 5794
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.23 ceramide + H2O Homo sapiens
-
 sphingosine + fatty acid
-
 ?
3.5.1.23 dihydroceramide + H2O Homo sapiens
-
 dihydrosphingosine + fatty acid
-
 ?
3.5.1.23 additional information Homo sapiens ACER3 can catalyze the hydrolysis of unsaturated long-chain dihydroceramides as efficiently as unsaturated long-chain ceramides. ACER1 and ACER2, the homologues of ACER3, also catalyzed the hydrolysis of certain dihydroceramide species to generate dihydrosphingosine ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Homo sapiens
-
 ACER1-3
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 HSC-1 cell skin squamous cell carcinoma cells, express very low levels of ACER3 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 ceramide + H2O
-
 Homo sapiens sphingosine + fatty acid
-
 ?
3.5.1.23 dihydroceramide + H2O
-
 Homo sapiens dihydrosphingosine + fatty acid
-
 ?
3.5.1.23 additional information ACER3 can catalyze the hydrolysis of unsaturated long-chain dihydroceramides as efficiently as unsaturated long-chain ceramides. ACER1 and ACER2, the homologues of ACER3, also catalyzed the hydrolysis of certain dihydroceramide species to generate dihydrosphingosine Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 (dihydro)ceramidase
-
 Homo sapiens
3.5.1.23 ACER-2
-
 Homo sapiens
3.5.1.23 ACER1
-
 Homo sapiens
3.5.1.23 ACER2
-
 Homo sapiens
3.5.1.23 ACER3
-
 Homo sapiens
3.5.1.23 alkaline ceramidase 2
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
3.5.1.23 Homo sapiens the retinoid N-(4-hydroxyphenyl)retinamide, i.e. fenretinide, increases the expression of ACER-2 through a retinoic acid receptor-independent and caspase-dependent manner up

General Information

EC Number General Information Comment Organism
3.5.1.23 metabolism inverse regulation of ACER2 and dihydrosphingosine desaturase is an important mechanism by which N-(4-hydroxyphenyl)retinamide exerts its cytotoxic and apoptotic effects in tumor cells Homo sapiens
3.5.1.23 physiological function ACER2 catalyzes the hydrolysis of dihydroceramides to generate dihydrosphingosine. ACER2 upregulation plays a key role in mediating the N-(4-hydroxyphenyl)retinamide-induced generation of dihydrosphingosine as well as the cytotoxicity of 4-HPR in tumor cells, while ACER3 has a limited role, overview Homo sapiens