EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | expressed in Escherichia coli BL21(DE3) cells | Human gammaherpesvirus 4 |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.23 | vapor diffusion method, using 0.3 M ammonium sulfate, 25% (w/v) polyethylene glycol 3350, 0.1 M HEPES, pH 7.0 (for mutant enzymes D131S/G78D and D131N), or using 150 mM malic acid, pH 7.5, and 25% (w/v) PEG 3350 (for mutant enzyme C4S), or using 0.1 M Tris, pH 8.5, 20% (w/v) polyethylene glycol 3350, 200 mM Li2SO4 (for mutant enzyme DELTAV, a 257-residue construct with the last 22 C-terminal residues removed) | Human gammaherpesvirus 4 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.23 | C4S | the mutant shows increased activity compared to the wild type enzyme | Human gammaherpesvirus 4 |
3.6.1.23 | D131E | the mutant is completely devoid of enzymatic activity | Human gammaherpesvirus 4 |
3.6.1.23 | D131N | the mutant shows decreased activity compared to the wild type enzyme | Human gammaherpesvirus 4 |
3.6.1.23 | D131S | the mutant shows decreased activity compared to the wild type enzyme | Human gammaherpesvirus 4 |
3.6.1.23 | D131S/G78D | the mutant shows decreased activity compared to the wild type enzyme | Human gammaherpesvirus 4 |
3.6.1.23 | F273A | the deletion of the flexible C-terminal tail carrying motif V results in a protein completely devoid of enzymatic activity | Human gammaherpesvirus 4 |
3.6.1.23 | R268A | the mutant shows decreased activity compared to the wild type enzyme | Human gammaherpesvirus 4 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.0006 | - |
dUTP | mutant enzyme C4S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.00071 | - |
dUTP | wild type enzyme, without MgCl2, with dithiothreitol, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.0008 | - |
dUTP | wild type enzyme, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.0052 | - |
dUTP | mutant enzyme D131S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.006 | - |
dUTP | wild type enzyme, without MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.007 | - |
dUTP | mutant enzyme D131N, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.008 | - |
dUTP | mutant enzyme R268A, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.009 | - |
dUTP | mutant enzyme D131S/G78D, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required, Mg2+ plays a role mainly in substrate binding | Human gammaherpesvirus 4 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Human gammaherpesvirus 4 | P03195 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.23 | Ni-NTA column chromatography | Human gammaherpesvirus 4 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | - |
Human gammaherpesvirus 4 | dUMP + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.23 | monomer | - |
Human gammaherpesvirus 4 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.23 | deoxyuridine 5'-triphosphate | - |
Human gammaherpesvirus 4 |
3.6.1.23 | dUTPase | - |
Human gammaherpesvirus 4 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.04 | - |
dUTP | mutant enzyme D131N, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.04 | - |
dUTP | mutant enzyme D131S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.06 | - |
dUTP | mutant enzyme R268A, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 0.18 | - |
dUTP | mutant enzyme D131S/G78D, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 1.2 | - |
dUTP | wild type enzyme, without MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 1.4 | - |
dUTP | wild type enzyme, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 1.6 | - |
dUTP | wild type enzyme, without MgCl2, with dithiothreitol, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 1.7 | - |
dUTP | mutant enzyme C4S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 6 | - |
dUTP | mutant enzyme D131N, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 8 | - |
dUTP | mutant enzyme D131S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 8 | - |
dUTP | mutant enzyme R268A, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 20 | - |
dUTP | mutant enzyme D131S/G78D, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 200 | - |
dUTP | wild type enzyme, without MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 1800 | - |
dUTP | wild type enzyme, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 2300 | - |
dUTP | wild type enzyme, without MgCl2, with dithiothreitol, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 | |
3.6.1.23 | 2800 | - |
dUTP | mutant enzyme C4S, with 1 mM MgCl2, in 0.5 mM bicine-NaOH, pH 7.8, at 25°C | Human gammaherpesvirus 4 |