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Literature summary extracted from

  • Pande, S.; Jahn, D.; Söll, D.
    Histidine tRNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and physical properties (1991), J. Biol. Chem., 266, 22826-22831.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.79 the enzyme shows a drastically shortened half-life in the absence of divalent metal ions Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.79 5,5'-dithiobis(2-nitrobenzoic)acid progressive loss of activity with increasing amounts of DTNB. Thus, sulfhydryl groups are involved in maintaining the active state of the enzyme or are involved in the mechanism Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.79 Co2+ the requirement for a divalent metal ion can be satisfied by Mg2+, Mn2+, and to a lesser extent by Co2+ and Zn2+. Activity in presence of 10 mM Co2+ is 13% compared to the activity in presence of 10 mM Mg2+ Saccharomyces cerevisiae
2.7.7.79 Mg2+ the requirement for a divalent metal ion can be satisfied by Mg2+, Mn2+, and to a lesser extent by Co2+ and Zn2+. Maximal activity by 10 mM Mg2+ Saccharomyces cerevisiae
2.7.7.79 Mn2+ the requirement for a divalent metal ion can be satisfied by Mg2+, Mn2+, and to a lesser extent by Co2+ and Zn2+. Activity in presence of 10 mM Mn2+ is 70% compared to the activity in presence of 10 mM Mg2+ Saccharomyces cerevisiae
2.7.7.79 Zn2+ the requirement for a divalent metal ion can be satisfied by Mg2+, Mn2+, and to a lesser extent by Co2+ and Zn2+. Activity in presence of 10 mM Zn2+ is 15% compared to the activity in presence of 10 mM Mg2+ Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.79 58000
-
 2 * 58000, SDS-PAGE Saccharomyces cerevisiae
2.7.7.79 120000
-
 gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.79 p-tRNAHis + ATP + GTP Saccharomyces cerevisiae
-
 pppGp-tRNAHis + AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.79 Saccharomyces cerevisiae P53215
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.79
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.79 p-tRNAHis + ATP + GTP
-
 Saccharomyces cerevisiae pppGp-tRNAHis + AMP + diphosphate
-
 ?
2.7.7.79 p-tRNAHis + ATP + GTP among all acceptor tRNAs in unfractionatedt RNA only tRNAHis is a substrate for the purified enzyme. tRNA from plant and prokaryotes are better substrates than mammalian and insect tRNAs Saccharomyces cerevisiae pppGp-tRNAHis + AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.79 dimer 2 * 58000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.7.7.79 histidine tRNA guanylyltransferase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.79 22
-
 assay at room temperature Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.79 8
-
 assay at Saccharomyces cerevisiae