Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hirai, K.; Kuroyanagi, H.; Tatebayashi, Y.; Hayashi, Y.; Hirabayashi-Takahashi, K.; Saito, K.; Haga, S.; Uemura, T.; Izumi, S.
    Dual role of the carboxyl-terminal region of pig liver L-kynurenine 3-monooxygenase: mitochondrial-targeting signal and enzymatic activity (2010), J. Biochem., 148, 639-650.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.9 DNA and amino acid sequence determnination and analysis, sequence comparisons, expression of FLAG-tagged wild-type enzyme and of C-terminal truncation mutants in COS-7 cells. The FLAG tag directs the wild-type enzyme to mitochondria but also to the cytosol and the plasma mambrane, overview Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.9 additional information construction of C-terminal truncation mutants KMODELTAC10, KMODELTAC20, KMODELTAC30, KMODELTAC50, and KMODELTAC70, that are all localized in the cytosol after recombinant expression in COS-7 cells, exept for KMODELTAC10. Two forms of C-terminal truncation, KMODELTAC10D and KMODELTAC20D retain almost full activity but KMODELTAC30D shows 1.6fold higher activity than the wild-type. The activities of KMODELTAC50 and KMODELTAC70 is highly reduced, overview. The quantity of expressed KMODELTA30D in COS-7 cellsis 1.3fold higher than the wild-type KMO Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.9 mitochondrial outer membrane the C-terminal region functions as a mitochondrial targeting signal Sus scrofa 5741
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.9 L-kynurenine + NADH + H+ + O2 Sus scrofa
-
 3-hydroxy-L-kynurenine + NAD+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + H+ + O2 Sus scrofa
-
 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.9 Sus scrofa Q9MZS9
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.9 liver
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.9 L-kynurenine + NADH + H+ + O2
-
 Sus scrofa 3-hydroxy-L-kynurenine + NAD+ + H2O
-
 ?
1.14.13.9 L-kynurenine + NADPH + H+ + O2
-
 Sus scrofa 3-hydroxy-L-kynurenine + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.9 More the C-terminal region is required for the enzymatic activity and functions as a mitochondrial targeting signal Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.14.13.9 KMO
-
 Sus scrofa
1.14.13.9 L-kynurenine 3-monooxygenase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.9 37
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.9 8
-
 assay at Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.9 FAD
-
 Sus scrofa
1.14.13.9 NADH
-
 Sus scrofa
1.14.13.9 NADPH
-
 Sus scrofa

General Information

EC Number General Information Comment Organism
1.14.13.9 additional information the C-terminal region of pig liver KMO plays a dual role. First, it is required for the enzymatic activity. Second, it functions as a mitochondrial targeting signal Sus scrofa