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Literature summary extracted from

  • Chagas, R.F.; Bailao, A.M.; Fernandes, K.F.; Winters, M.S.; Pereira, M.; Soares, C.M.
    Purification of Paracoccidioides brasiliensis catalase P: subsequent kinetic and stability studies (2010), J. Biochem., 147, 345-351.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.6 NADPH addition of NADPH stimulates the activity of CatP in a dose-dependent manner most likely by preventing the inactivation from oxidative damage through its substrate H2O2 Paracoccidioides brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-Amino-1,2,4-triazole specific irreversible inhibitor, complete inhibition at 20 mM Paracoccidioides brasiliensis
1.11.1.6 azide irreversible inhibitor, effective at concentrations less than1 mM Paracoccidioides brasiliensis
1.11.1.6 hydroxylamine
-
 Paracoccidioides brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 61000
-
 4 * 61000, SDS-PAGE Paracoccidioides brasiliensis
1.11.1.6 244000
-
 gel filtration Paracoccidioides brasiliensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.11.1.6 guanidine-HCl CatP is highly resistant to denaturation by guanidine-HCl. The enzyme conserves activity after 48 h exposure to 1 M guanidine-HCl. Guanidine-HCl at 1.5 M for 48 h decreases the catalase P activity to about 60% Paracoccidioides brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Paracoccidioides brasiliensis Q8X220
-
-
1.11.1.6 Paracoccidioides brasiliensis ATCC MYA-826 Q8X220
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 acetone precipitation and Sephadex G-150 gel filtration Paracoccidioides brasiliensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 366.6
-
 crude extract, in 50 mM sodium phosphate buffer, pH 7.8, at 37°C Paracoccidioides brasiliensis
1.11.1.6 1134
-
 after 3.1fold purification, in 50 mM sodium phosphate buffer, pH 7.8, at 37°C Paracoccidioides brasiliensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Paracoccidioides brasiliensis O2 + H2O
-
 ?
1.11.1.6 H2O2
-
 Paracoccidioides brasiliensis ATCC MYA-826 O2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 homotetramer 4 * 61000, SDS-PAGE Paracoccidioides brasiliensis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 catalase P
-
 Paracoccidioides brasiliensis
1.11.1.6 CatP
-
 Paracoccidioides brasiliensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 4 12 the purified CatP is stable and yields similar activity from pH 4.0 to 12.0 Paracoccidioides brasiliensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.6 3.8 7.8 approximately 20% activity is observed at pH 3.8 followed by a steady rise of activity as the pH is increased to 7.8 (100% activity) Paracoccidioides brasiliensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.6 Paracoccidioides brasiliensis isoelectric focusing
-
 6.2

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.11.1.6 0.0015
-
 in 50 mM sodium phosphate buffer, pH 7.8, at 37°C Paracoccidioides brasiliensis azide
1.11.1.6 0.002
-
 in 50 mM sodium phosphate buffer, pH 7.8, at 37°C Paracoccidioides brasiliensis hydroxylamine

Expression

EC Number Organism Comment Expression
1.11.1.6 Paracoccidioides brasiliensis CatP presents high levels of expression in the yeast parasitic phase up