EC Number | Cloned (Comment) | Organism |
---|---|---|
6.2.1.27 | - |
Thauera aromatica |
6.2.1.37 | - |
Thauera aromatica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.2.1.27 | 0.065 | - |
4-hydroxybenzoate | pH 7.8, 30°C | Thauera aromatica | |
6.2.1.37 | 0.06 | - |
3-hydroxybenzoate | pH 7.8, 30°C | Thauera aromatica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 60000 | - |
gel filtration | Thauera aromatica |
6.2.1.37 | 60000 | - |
gel filtration | Thauera aromatica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | Thauera aromatica | the enzyme is involved in the anaerobic hydroxybenzoate metabolism | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | Thauera aromatica | the enzyme is involved in the anaerobic hydroxybenzoate metabolism | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.2.1.27 | Thauera aromatica | Q9AJS8 | - |
- |
6.2.1.37 | Thauera aromatica | Q9AJS8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.2.1.27 | - |
Thauera aromatica |
6.2.1.37 | - |
Thauera aromatica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 4.12 | - |
pH 7.8, 30°C | Thauera aromatica |
6.2.1.37 | 4.12 | - |
pH 7.8, 30°C | Thauera aromatica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | the enzyme is involved in the anaerobic hydroxybenzoate metabolism | Thauera aromatica | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | ATP + 4-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 3-hydroxybenzoate (cf. EC 6.2.1.37). Activity with 4-hydroxybenzoate is 83% of the activity with 3-hydroxybenzoate | Thauera aromatica | AMP + diphosphate + 4-hydroxybenzoyl-CoA | - |
? | |
6.2.1.27 | additional information | the enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4-chlorobenzoate. There is no activity with 3,4-dihydroxybenzoate, 2,3-dihydroxybenzoate, and 2-hydroxybenzoate as substrates | Thauera aromatica | ? | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | the enzyme is involved in the anaerobic hydroxybenzoate metabolism | Thauera aromatica | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | ATP + 3-hydroxybenzoate + CoA | the bifunctional enzyme is also active with 4-hydroxybenzoate (cf. EC 6.2.1.27). Activity with 4-hydroxybenzoate is 83% of the activity with 3-hydroxybenzoate | Thauera aromatica | AMP + diphosphate + 3-hydroxybenzoyl-CoA | - |
? | |
6.2.1.37 | additional information | the enzyme shows low activity towards benzoate, 4-aminobenzoate, 3-aminobenzoate, 3-fluorobenzoate, 4-fluorobenzoate, 3-chlorobenzoate, and 4-chlorobenzoate. There is no activity with 3,4-dihydroxybenzoate, 2,3-dihydroxybenzoate, and 2-hydroxybenzoate as substrates | Thauera aromatica | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.2.1.27 | monomer | 1 * 60000, SDS-PAGE | Thauera aromatica |
6.2.1.37 | monomer | 1 * 60000, SDS-PAGE | Thauera aromatica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.2.1.37 | 3-hydroxybenzoate-coenzyme A ligase (AMP-forming) | - |
Thauera aromatica |
6.2.1.37 | 3-hydroxybenzoyl coenzyme A synthetase | - |
Thauera aromatica |
6.2.1.37 | 3-hydroxybenzoyl-CoA ligase | - |
Thauera aromatica |
6.2.1.37 | 3-hydroxybenzoyl-CoA synthetase | - |
Thauera aromatica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 9 | - |
or above. At a pH of above 9 the coupled spectrophotometric assay becomes limited due to instability of the substrates | Thauera aromatica |
6.2.1.37 | 9 | - |
or above. At a pH of above 9 the coupled spectrophotometric assay becomes limited due to instability of the substrates | Thauera aromatica |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.2.1.27 | 7 | 9 | pH 7.0: 20% of the activity at pH 9, pH 8.0: 65% of the activity at pH 9 | Thauera aromatica |
6.2.1.37 | 7 | 9 | pH 7.0: 20% of the activity at pH 9, pH 8.0: 65% of the activity at pH 9 | Thauera aromatica |
EC Number | Organism | Comment | Expression |
---|---|---|---|
6.2.1.27 | Thauera aromatica | induction during anaerobic growth with by 3-hydroxybenzoate | up |
6.2.1.37 | Thauera aromatica | induction during anaerobic growth with by 3-hydroxybenzoate | up |