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Literature summary extracted from

  • Kapoor, N.; Banerjee, T.; Babu, P.; Maity, K.; Surolia, N.; Surolia, A.
    Design, development, synthesis, and docking analysis of 2-substituted triclosan analogs as inhibitors for Plasmodium falciparum enoyl-ACP reductase (2009), IUBMB Life, 61, 1083-1091.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9 expression of the His6-tagged ENR in Escherichia coli BL21 Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 2-(2'-amino-4'-chloro-phenoxy)-5-chloro-phenol inhibits the parasite growth, uncompetitive inhibition kinetics with crotonoyl-CoA and competitive with NADH Plasmodium falciparum
1.3.1.9 4-chloro-1-(4-chloro-2-methoxyphenoxy)-2-nitrobenzene
-
 Plasmodium falciparum
1.3.1.9 4-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-4-oxobutanoic acid
-
 Plasmodium falciparum
1.3.1.9 4-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-4-oxobutanoic acid
-
 Plasmodium falciparum
1.3.1.9 5-chloro-2-(4'-chloro-2'-nitro-phenoxy)-phenol inhibits the parasite growth Plasmodium falciparum
1.3.1.9 5-chloro-2-(4-chloro-2-methoxyphenoxy)aniline
-
 Plasmodium falciparum
1.3.1.9 5-chloro-2-[4-chloro-2-(prop-1-en-2-ylamino)phenoxy]phenol
-
 Plasmodium falciparum
1.3.1.9 5-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-5-oxopentanoic acid
-
 Plasmodium falciparum
1.3.1.9 5-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-5-oxopentanoic acid
-
 Plasmodium falciparum
1.3.1.9 6-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-6-oxohexanoic acid
-
 Plasmodium falciparum
1.3.1.9 6-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-6-oxohexanoic acid
-
 Plasmodium falciparum
1.3.1.9 additional information structure-based approach for development of 2'-substituted analogues of triclosan as enzyme inhibitors, binding energies and IC50 in culture, overview Plasmodium falciparum
1.3.1.9 N-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]acetamide
-
 Plasmodium falciparum
1.3.1.9 triclosan
-
 Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.9 an acyl-[acyl-carrier protein] + NAD+ Plasmodium falciparum
-
 a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.9 recombinant His6-tagged ENR from Escherichia coli BL21 by nickel affinity chromatography Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 an acyl-[acyl-carrier protein] + NAD+
-
 Plasmodium falciparum a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH + H+
-
 ?
1.3.1.9 additional information activity detection via measurement of NADPH production by reduction of crotonoyl-CoA Plasmodium falciparum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 enoyl-ACP reductase
-
 Plasmodium falciparum
1.3.1.9 enoyl-acyl carrier protein reductase
-
 Plasmodium falciparum
1.3.1.9 ENR
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.9 25
-
 assay at Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.9 7.5
-
 assay at Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.9 NAD+
-
 Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.9 0.000096
-
 2-(2'-amino-4'-chloro-phenoxy)-5-chloro-phenol versus NADP+, pH 7.5, 25°C Plasmodium falciparum
1.3.1.9 0.000104
-
 2-(2'-amino-4'-chloro-phenoxy)-5-chloro-phenol versus crotonoyl-CoA-[acyl-carrier protein], pH 7.5, 25°C Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.3.1.9 0.000073
-
 pH 7.5, 25°C Plasmodium falciparum triclosan
1.3.1.9 0.00011
-
 pH 7.5, 25°C Plasmodium falciparum 2-(2'-amino-4'-chloro-phenoxy)-5-chloro-phenol
1.3.1.9 0.000229
-
 pH 7.5, 25°C Plasmodium falciparum 5-chloro-2-(4'-chloro-2'-nitro-phenoxy)-phenol
1.3.1.9 0.01
-
 pH 7.5, 25°C Plasmodium falciparum 4-chloro-1-(4-chloro-2-methoxyphenoxy)-2-nitrobenzene
1.3.1.9 0.0122
-
 pH 7.5, 25°C Plasmodium falciparum N-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]acetamide
1.3.1.9 0.015
-
 pH 7.5, 25°C Plasmodium falciparum 5-chloro-2-(4-chloro-2-methoxyphenoxy)aniline
1.3.1.9 0.02
-
 pH 7.5, 25°C Plasmodium falciparum 5-chloro-2-[4-chloro-2-(prop-1-en-2-ylamino)phenoxy]phenol
1.3.1.9 0.0224
-
 pH 7.5, 25°C Plasmodium falciparum 4-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-4-oxobutanoic acid
1.3.1.9 0.0242
-
 pH 7.5, 25°C Plasmodium falciparum 4-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-4-oxobutanoic acid
1.3.1.9 0.026
-
 pH 7.5, 25°C Plasmodium falciparum 5-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-5-oxopentanoic acid
1.3.1.9 0.0286
-
 pH 7.5, 25°C Plasmodium falciparum 5-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-5-oxopentanoic acid
1.3.1.9 0.0293
-
 pH 7.5, 25°C Plasmodium falciparum 6-[5-chloro-2-(4-chloro-2-hydroxyphenoxy)phenyl]amino-6-oxohexanoic acid
1.3.1.9 0.0303
-
 pH 7.5, 25°C Plasmodium falciparum 6-[5-chloro-2-(4-chloro-2-methoxyphenoxy)phenyl]amino-6-oxohexanoic acid

General Information

EC Number General Information Comment Organism
1.3.1.9 physiological function PfENR reduces the trans-2 enoyl bond of enoyl-ACP substrates to saturated acyl-ACPs and plays a crucial role in completing the successive rounds of fatty acid elongation Plasmodium falciparum