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Literature summary extracted from

  • Mu, H.; Zhou, S.M.; Xia, Y.; Zou, H.; Meng, F.; Yan, Y.B.
    Inactivation and unfolding of the hyperthermophilic inorganic pyrophosphatase from Thermus thermophilus by sodium dodecyl sulfate (2009), Int. J. Mol. Sci., 10, 2849-2859.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.1 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.1 SDS SDS acts as a mixed-type reversible inhibitor of PPase, PPase can be fully inactivated at SDS concentration of 2 mM Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.1 Thermus thermophilus P38576
-
-
3.6.1.1 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 P38576
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.1 Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.1 diphosphate + H2O
-
 Thermus thermophilus phosphate + phosphate
-
 ?
3.6.1.1 diphosphate + H2O
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.1 inorganic pyrophosphatase
-
 Thermus thermophilus
3.6.1.1 PPase
-
 Thermus thermophilus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.1 1.11
-
 at 25°C, pH not specified in the publication Thermus thermophilus SDS

General Information

EC Number General Information Comment Organism
3.6.1.1 metabolism PPase is an essential constitutive enzyme for energy metabolism and clearance of excess diphosphate Thermus thermophilus