Literature summary extracted from

Borges, C.L.; Parente, J.A.; Barbosa, M.S.; Santana, J.M.; Bao, S.N.; de Sousa, M.V.; de Almeida Soares, C.M.
Detection of a homotetrameric structure and protein-protein interactions of Paracoccidioides brasiliensis formamidase lead to new functional insights (2010), FEMS Yeast Res., 10, 104-113.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.5.1.49	expressed in Escherichia coli BL21(DE3) cells	Paracoccidioides brasiliensis

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.5.1.49	cell wall	-	Paracoccidioides brasiliensis	5618	-
3.5.1.49	cytoplasm	-	Paracoccidioides brasiliensis	5737	-

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.5.1.49	43300	-	4 * 43300, native state, native PAGE	Paracoccidioides brasiliensis
3.5.1.49	45000	-	4 * 45000, SDS-PAGE	Paracoccidioides brasiliensis
3.5.1.49	180000	-	SDS-PAGE, the 180000 Da purified protein yields a heat-denatured species of 45000 Da	Paracoccidioides brasiliensis
3.5.1.49	191000	-	purified formamidase, native PAGE	Paracoccidioides brasiliensis

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.5.1.49	Paracoccidioides brasiliensis	C1GWV9	isolate 01 (ATCC MYA-826)	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.5.1.49	glutatione Sepharose column chromatography, DEAE Sepharose column chromatography, and phenyl Sepharose column chromatography	Paracoccidioides brasiliensis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.5.1.49	formamide + H2O	-	Paracoccidioides brasiliensis	formate + NH3	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.5.1.49	homotetramer	4 * 45000, SDS-PAGE	Paracoccidioides brasiliensis
3.5.1.49	homotetramer	4 * 43300, native state, native PAGE	Paracoccidioides brasiliensis

Synonyms

EC Number	Synonyms	Comment	Organism
3.5.1.49	Fmd	-	Paracoccidioides brasiliensis
3.5.1.49	formamide aminohydrolase	-	Paracoccidioides brasiliensis

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Release 2023.1 (January 2023)