EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | L-aspartate | 10fold activation | Trypanosoma cruzi | |
1.1.1.40 | L-aspartate | the cytosolic isozyme is 50% activated by L-aspartate | Trypanosoma brucei | |
1.1.1.40 | L-aspartate | the cytosolic isozyme is over 10fold activated by L-aspartate | Trypanosoma cruzi |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.40 | cytosolic and mitochondrial isozymes, expression in of His-tagged isozymes ME1 and ME2 Escherichia coli Rosetta (DE3)pLysS | Trypanosoma brucei |
1.1.1.40 | cytosolic and mitochondrial isozymes, expression of His-tagged ME2 in Escherichia coli Rosetta (DE3)pLysS and of His-tagged ME1 in Escherichia coli BL(DE3) | Trypanosoma cruzi |
1.1.1.40 | expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3) | Trypanosoma brucei |
1.1.1.40 | expression of His-tagged isozymes ME1 and ME2 in Escherichia coli strain BL21(DE3) | Trypanosoma cruzi |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.40 | recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography | Trypanosoma brucei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | oxaloacetate | competitive | Trypanosoma brucei | |
1.1.1.40 | oxaloacetate | competitive | Trypanosoma cruzi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 0.016 | - |
NADP+ | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 0.016 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 0.027 | - |
NADP+ | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 0.027 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 0.032 | - |
NADP+ | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 0.032 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 0.048 | - |
NADP+ | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 0.048 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 0.13 | - |
(S)-malate | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 0.13 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 0.32 | - |
(S)-malate | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 0.32 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 0.49 | - |
(S)-malate | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 0.49 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 2.3 | - |
(S)-malate | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 2.3 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 4.3 | - |
pyruvate | isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 4.3 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 5.1 | - |
pyruvate | isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 5.1 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 5.3 | - |
pyruvate | isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 5.3 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 7.6 | - |
pyruvate | isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 7.6 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.40 | cytosol | cytosolic isozyme, appears to be clearly more abundant in the insect stage, although it can be immunodetected in the bloodstream form | Trypanosoma brucei | 5829 | - |
1.1.1.40 | cytosol | cytosolic isozyme, the cytosolic isoform appears to be more abundant in the insect stages of the parasite | Trypanosoma cruzi | 5829 | - |
1.1.1.40 | cytosol | both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms | Trypanosoma brucei | 5829 | - |
1.1.1.40 | cytosol | the cytosolic isoform is more abundant in the insect stages of the parasite | Trypanosoma cruzi | 5829 | - |
1.1.1.40 | mitochondrion | mitochondrial isozyme, appears to be clearly more abundant in the insect stage, although it can be immunodetected in the bloodstream form | Trypanosoma brucei | 5739 | - |
1.1.1.40 | mitochondrion | mitochondrial isozyme, the expression of the mitochondrial isozyme seems to be clearly upregulated in amastigotes | Trypanosoma cruzi | 5739 | - |
1.1.1.40 | mitochondrion | both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms | Trypanosoma brucei | 5739 | - |
1.1.1.40 | mitochondrion | expression of the mitochondrial ME is upregulated in amastigotes | Trypanosoma cruzi | 5739 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | Mg2+ | activates, kcat is 1280/s and Km 0.27 mM for ME1, and kcat is 208/s and Km 0.58 mM for ME2 | Trypanosoma brucei | |
1.1.1.40 | Mg2+ | highly activating, kcat is 461/s and Km 0.04 mM for ME1, and kcat is 864/s and Km 0.57 mM for ME2 | Trypanosoma cruzi | |
1.1.1.40 | Mg2+ | can partially substitute for Mn2+, activates | Trypanosoma brucei | |
1.1.1.40 | Mg2+ | can partially substitute for Mn2+, activates | Trypanosoma cruzi | |
1.1.1.40 | Mn2+ | activates, required | Trypanosoma brucei | |
1.1.1.40 | Mn2+ | activates, required | Trypanosoma cruzi | |
1.1.1.40 | Mn2+ | activates, kcat is 518/s and Km 0.0043 mM for ME1, and kcat is 687/s and Km 0.0091 mM for ME2 | Trypanosoma cruzi | |
1.1.1.40 | Mn2+ | highly activating, kcat is 905/s and Km 0.0024 mM for ME1, and kcat is 246/s and Km 0.0066 mM for ME2 | Trypanosoma brucei |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma brucei | - |
pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma cruzi | - |
pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma brucei | - |
pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma cruzi | - |
pyruvate + NADPH + H+ + CO2 | - |
? | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma cruzi CL Brener | - |
pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma cruzi CL Brener | - |
pyruvate + NADPH + H+ + CO2 | - |
? | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma brucei 427 | - |
pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma brucei 427 | - |
pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma brucei stock 427 | - |
pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | Trypanosoma brucei stock 427 | - |
pyruvate + NADPH + H+ + CO2 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.40 | Trypanosoma brucei | - |
- |
- |
1.1.1.40 | Trypanosoma brucei | - |
grown in male Wistar rats | - |
1.1.1.40 | Trypanosoma brucei 427 | - |
- |
- |
1.1.1.40 | Trypanosoma brucei stock 427 | - |
grown in male Wistar rats | - |
1.1.1.40 | Trypanosoma cruzi | - |
- |
- |
1.1.1.40 | Trypanosoma cruzi CL Brener | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.40 | recombinant His-tagged cytosolic and mitochondrial isozymes from Escherichia coli by nickel affinity chromatography | Trypanosoma cruzi |
1.1.1.40 | recombinant His-tagged isozymes ME1 and ME2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Trypanosoma brucei |
1.1.1.40 | recombinant His-tagged isozymes ME1 and ME2 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Trypanosoma cruzi |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.40 | amastigote | - |
Trypanosoma cruzi | - |
1.1.1.40 | epimastigote | - |
Trypanosoma cruzi | - |
1.1.1.40 | additional information | both isozymes appear to be clearly more abundant in the insect stage, although they can be immunodetected in the bloodstream forms | Trypanosoma brucei | - |
1.1.1.40 | additional information | the expression of the mitochondrial isozyme seems to be clearly upregulated in amastigotes, whereas the cytosolic isoform appears to be more abundant in the insect stages of the parasite | Trypanosoma cruzi | - |
1.1.1.40 | additional information | both cytosolic and mitochondrial isozymes are more abundant in the insect stage, although they can be immunodetected in the bloodstream forms | Trypanosoma brucei | - |
1.1.1.40 | additional information | expression of the mitochondrial ME is upregulated in amastigotes, while the cytosolic isoform is more abundant in the insect stages of the parasite | Trypanosoma cruzi | - |
1.1.1.40 | procyclic form | - |
Trypanosoma brucei | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma brucei | pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma cruzi | pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma cruzi CL Brener | pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma brucei 427 | pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma brucei stock 427 | pyruvate + CO2 + NADPH | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma brucei | pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma cruzi | pyruvate + NADPH + H+ + CO2 | - |
? | |
1.1.1.40 | (S)-malate + NADP+ | oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs | Trypanosoma brucei | pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs | Trypanosoma cruzi | pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma cruzi CL Brener | pyruvate + NADPH + H+ + CO2 | - |
? | |
1.1.1.40 | (S)-malate + NADP+ | oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs | Trypanosoma cruzi CL Brener | pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma brucei 427 | pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs | Trypanosoma brucei 427 | pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | - |
Trypanosoma brucei stock 427 | pyruvate + NADPH + H+ + CO2 | - |
r | |
1.1.1.40 | (S)-malate + NADP+ | oxaloacetate represents the intermediate resulting from dehydrogenation of malate during the first step of the catalytic cycle of MEs | Trypanosoma brucei stock 427 | pyruvate + NADPH + H+ + CO2 | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.40 | tetramer | - |
Trypanosoma brucei |
1.1.1.40 | tetramer | - |
Trypanosoma cruzi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.40 | malic enzyme | - |
Trypanosoma brucei |
1.1.1.40 | malic enzyme | - |
Trypanosoma cruzi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 8.3 | - |
pyruvate | isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 8.3 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 34 | - |
pyruvate | isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 34 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 39 | - |
pyruvate | isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 39 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 113 | - |
pyruvate | isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 113 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 242 | - |
(S)-malate | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 242 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 284 | - |
NADP+ | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 284 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 504 | - |
NADP+ | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 504 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 518 | - |
(S)-malate | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 518 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 683 | - |
(S)-malate | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 683 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 691 | - |
NADP+ | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 691 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 1132 | - |
(S)-malate | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 1132 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 1206 | - |
NADP+ | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 1206 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 7.4 | - |
assay at | Trypanosoma brucei |
1.1.1.40 | 7.4 | - |
assay at | Trypanosoma cruzi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | NADP+ | - |
Trypanosoma brucei | |
1.1.1.40 | NADP+ | - |
Trypanosoma cruzi | |
1.1.1.40 | NADP+ | specific for | Trypanosoma brucei | |
1.1.1.40 | NADP+ | specific for | Trypanosoma cruzi | |
1.1.1.40 | NADPH | - |
Trypanosoma brucei | |
1.1.1.40 | NADPH | - |
Trypanosoma cruzi | |
1.1.1.40 | NADPH | specific for | Trypanosoma brucei | |
1.1.1.40 | NADPH | specific for | Trypanosoma cruzi |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.1.1.40 | physiological function | in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power | Trypanosoma brucei |
1.1.1.40 | physiological function | in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power | Trypanosoma cruzi |
1.1.1.40 | physiological function | possibly, in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power | Trypanosoma brucei |
1.1.1.40 | physiological function | possibly, in those environments where glucose is very low or absent, the pathogen depends on NADP-linked dehydrogenases such as the MEs for NADPH production, as in those conditions the pentose phosphate pathway cannot serve as a source of essential reducing power | Trypanosoma cruzi |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.40 | 1.1 | - |
pyruvate | isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 1.1 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 6 | - |
pyruvate | isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 6 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 8 | - |
pyruvate | isozyme ME2, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 8 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 26 | - |
pyruvate | isozyme ME1, with NADPH and CO2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 26 | - |
pyruvate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 297 | - |
(S)-malate | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 297 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 494 | - |
(S)-malate | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 494 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 3536 | - |
(S)-malate | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 3536 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 3981 | - |
(S)-malate | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 3981 | - |
(S)-malate | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 17780 | - |
NADP+ | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 17780 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma brucei | |
1.1.1.40 | 18680 | - |
NADP+ | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 18680 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 21600 | - |
NADP+ | isozyme ME2, pH 7.4, temperature not specified in the publication | Trypanosoma cruzi | |
1.1.1.40 | 21600 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME2, in presence of 0.5 mM Mn2+ | Trypanosoma cruzi | |
1.1.1.40 | 25170 | - |
NADP+ | isozyme ME1, pH 7.4, temperature not specified in the publication | Trypanosoma brucei | |
1.1.1.40 | 25170 | - |
NADP+ | pH and temperature not specified in the publication, recombinant isozyme ME1, in presence of 0.5 mM Mn2+ | Trypanosoma brucei |