EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.10 | expressed in Escherichia coli BL21 Star (DE3) cells | Trametes ochracea |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.3.10 | mutant enzymes are crystallized by the hanging drop vapor diffusion method, using 10% (w/v) monomethyl ether poly(ethlene glycol) 2000 , 0.1 M MES (pH 5.2), 50 mM MgCl2 | Trametes ochracea |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.3.10 | F454A/S455A/Y456A | the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme | Trametes ochracea |
1.1.3.10 | F454A/Y456A | the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme | Trametes ochracea |
1.1.3.10 | F454N | the apparent kcat value with D-glucose and O2 (fixed at a concentration of 0.256 mM, air saturation) as substrates decreases dramatically compared to the wild type enzyme | Trametes ochracea |
1.1.3.10 | F454N | the mutant shows decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme | Trametes ochracea |
1.1.3.10 | F454P | the mutant shows strongly decreased catalytic efficiency for D-glucose/O2 compared to the wild type enzyme | Trametes ochracea |
1.1.3.10 | H450Q | the turnover number for the electron donor/acceptor substrate pair D-galactose/O2 is comparable to that of the wild type enzyme | Trametes ochracea |
1.1.3.10 | additional information | the deletion mutant DELTA454-456 shows 6fold lower kcat (D-galactose/O2), and 0.8fold elevated Km (D-galactose) value compared to the wild type enzyme | Trametes ochracea |
1.1.3.10 | Y456W | the turnover number for the electron donor/acceptor substrate pair D-galactose/O2 is comparable to that of the wild type enzyme | Trametes ochracea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.10 | 0.0052 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.0071 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.0089 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.01 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.013 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.015 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.016 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.023 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.025 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.027 | - |
1,4-benzoquinone | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.029 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.029 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.041 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.049 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.052 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.072 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.072 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.078 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.1 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.14 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.14 | - |
1,4-benzoquinone | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.15 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.24 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.24 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.28 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.35 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.38 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.4 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.76 | - |
D-glucose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.5 | - |
D-glucose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.5 | - |
D-glucose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.7 | - |
D-glucose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.8 | - |
D-glucose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2 | 3 | D-melibiose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2.1 | - |
D-glucose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2.5 | - |
D-glucose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 6.1 | - |
D-galactose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 10 | - |
D-galactose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 13 | - |
D-galactose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 13 | - |
D-galactose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 26 | - |
D-galactose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 29 | - |
D-galactose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 34 | - |
D-galactose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 50 | - |
D-melibiose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 240 | - |
D-melibiose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 260 | - |
D-melibiose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 350 | - |
D-melibiose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 390 | - |
D-melibiose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1500 | - |
D-melibiose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | 270000 | - |
gel filtration | Trametes ochracea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.10 | Trametes ochracea | Q7ZA32 | synonym Trametes ochracea | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.10 | Ni2+-immobilized metal affinity column chromatography | Trametes ochracea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.10 | 2-deoxy-2-fluoro-D-glucose + O2 | slow substrate | Trametes ochracea | 2-deoxy-3-dehydro-D-glucose + H2O2 | - |
? | |
1.1.3.10 | beta-D-galactose + O2 | poor substrate | Trametes ochracea | 2-dehydro-D-galactose + H2O2 | - |
? | |
1.1.3.10 | D-galactose + 1,4-benzoquinone | - |
Trametes ochracea | 2-dehydro-D-galactose + hydroquinone | - |
? | |
1.1.3.10 | D-galactose + ferrocenium hexafluorophosphate | - |
Trametes ochracea | 2-dehydro-D-galactose + ferrocene | - |
? | |
1.1.3.10 | D-glucose + 1,4-benzoquinone | 1,4-benzoquinone is a physiologically relevant alternative electron acceptor in the oxidative half-reaction | Trametes ochracea | 2-dehydro-D-glucose + hydroquinone | - |
? | |
1.1.3.10 | D-glucose + ferrocenium hexafluorophosphate | - |
Trametes ochracea | 2-dehydro-D-glucose + ferrocene | - |
? | |
1.1.3.10 | D-glucose + O2 | the wild type enzyme displays a clear preference for D-glucose over D-galactose | Trametes ochracea | 2-dehydro-D-glucose + H2O2 | - |
? | |
1.1.3.10 | D-melibiose + O2 | - |
Trametes ochracea | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.10 | homotetramer | 2 * 000, SDS-PAGE | Trametes ochracea |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.3.10 | P2O | - |
Trametes ochracea |
1.1.3.10 | pyranose 2-Oxidase | - |
Trametes ochracea |
1.1.3.10 | pyranose:oxygen 2-oxidoreductase | - |
Trametes ochracea |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.3.10 | 60 | 70 | the half life of the wild type enzyme at 60°C and 70°C is 12 min and 0.07 min, respectively. The melting temperature of the wild type enzyme is at 63.5°C | Trametes ochracea |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.10 | 0.14 | - |
D-galactose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.19 | - |
D-melibiose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.2 | - |
D-glucose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.29 | - |
D-melibiose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.3 | - |
D-galactose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.64 | - |
D-galactose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.99 | - |
D-glucose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.2 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.2 | - |
D-galactose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.3 | - |
D-melibiose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.5 | - |
D-galactose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1.7 | - |
D-galactose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2 | - |
D-galactose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2.7 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2.7 | - |
D-melibiose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2.9 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2.9 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 3 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 3.3 | - |
D-melibiose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 3.3 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 3.8 | - |
1,4-benzoquinone | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 4.4 | - |
D-melibiose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 4.5 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 5.4 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 6.6 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 7.1 | - |
D-glucose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 7.3 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 7.3 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 7.6 | - |
D-melibiose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 12 | - |
D-glucose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 15 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 17 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 17 | - |
D-glucose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 26 | - |
D-glucose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 30 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 33 | - |
D-glucose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 61 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 67 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 74 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 110 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 130 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 160 | - |
1,4-benzoquinone | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 210 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 220 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 220 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 400 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 420 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 470 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.10 | FAD | - |
Trametes ochracea |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.10 | 0.0037 | - |
D-melibiose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.005 | - |
D-melibiose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.0058 | - |
D-melibiose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.008 | - |
D-melibiose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.0084 | - |
D-melibiose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.011 | - |
D-galactose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.0113 | - |
D-melibiose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.0166 | - |
D-melibiose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.024 | - |
D-galactose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.046 | - |
D-galactose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.053 | - |
D-galactose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.059 | - |
D-galactose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.062 | - |
D-galactose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.094 | - |
D-glucose | mutant enzyme F454A/S455A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.27 | - |
D-galactose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 0.54 | - |
D-glucose | mutant enzyme F454P, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 4.7 | - |
D-glucose | mutant enzyme F454A/Y456A, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 7 | - |
D-glucose | mutant enzyme H450Q, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 8.2 | - |
D-glucose | mutant enzyme F454N, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 15 | - |
D-glucose | mutant enzyme Y456W, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 37 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 43 | - |
D-glucose | wild type enzyme, using O2 as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 63 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 130 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 140 | - |
1,4-benzoquinone | wild type enzyme, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 180 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 180 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 200 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 230 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 280 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 290 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 330 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 340 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 360 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 370 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 420 | - |
1,4-benzoquinone | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 480 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454P, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 500 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 510 | - |
ferrocenium hexafluorophosphate | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 520 | - |
1,4-benzoquinone | mutant enzyme F454A/S455A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 520 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-galactose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 940 | - |
1,4-benzoquinone | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1200 | - |
1,4-benzoquinone | wild type enzyme, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1210 | - |
ferrocenium hexafluorophosphate | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1250 | - |
ferrocenium hexafluorophosphate | mutant enzyme H450Q, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 1640 | - |
ferrocenium hexafluorophosphate | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2100 | - |
1,4-benzoquinone | mutant enzyme F454A/Y456A, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 2600 | - |
1,4-benzoquinone | mutant enzyme F454N, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea | |
1.1.3.10 | 3000 | - |
1,4-benzoquinone | mutant enzyme Y456W, using D-glucose as cosubstrate, in 50 mM KH2PO4 buffer (pH 6.5), at 30°C | Trametes ochracea |