EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.4.25 | Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I | the mutant shows a 2fold increase in GTP cyclohydrolase II activity and a 4fold increase in the Km value with GTP as the substrate. Using 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate as the substrate, the mutant shows a rate enhancement by a factor of about 2 and an increase in the Km value by a factor of about 5 | Bacillus subtilis |
3.5.4.25 | Y210C/A290T/Q293R/A296T/K322R/M361I | the mutant shows increased kcat and Km values compared to the wild type enzyme using GTP and 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate as the substrate | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.25 | 0.01 | - |
GTP | wild type enzyme, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.031 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | wild type enzyme, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.044 | - |
GTP | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.049 | - |
GTP | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.079 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.122 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.25 | Bacillus subtilis | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.25 | 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate + H2O | - |
Bacillus subtilis | ? | - |
? | |
3.5.4.25 | GTP + H2O | - |
Bacillus subtilis | formate + 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.4.25 | GCH II | - |
Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.4.25 | 0.035 | - |
GTP | wild type enzyme, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.05 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | wild type enzyme, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.065 | - |
GTP | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.072 | - |
GTP | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.09 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/M361I, at pH 8.5 and 30°C | Bacillus subtilis | |
3.5.4.25 | 0.1 | - |
2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-triphosphate | mutant enzyme Y210C/A290T/Q293R/A296T/K322R/F339Y/M361I, at pH 8.5 and 30°C | Bacillus subtilis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.5.4.25 | metabolism | GTP cyclohydrolase II catalyzes the first dedicated step in the biosynthesis of riboflavin | Bacillus subtilis |