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Literature summary extracted from

  • Punj, S.; John, G.
    Purification and identification of an FMN-dependent NAD(P)H azoreductase from Enterococcus faecalis (2009), Curr. Issues Mol. Biol., 11, 59-66.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.011
-
 Methyl red in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis
1.7.1.6 0.082
-
 NADH in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis
1.7.1.6 15
-
 NADPH in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Enterococcus faecalis
-
-
-
1.7.1.6 Enterococcus faecalis ATCC 27274
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6 octyl Sepharose column chromatography, DEAE Bio-gel agarose column chromatography, and Affi-gel Blue gel filtration Enterococcus faecalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 0.003
-
 crude extract, using methyl red and NADPH as substrates, in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis
1.7.1.6 626
-
 after 209fold purification, using methyl red and NADPH as substrates, in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 methyl red + NADH + H+
-
 Enterococcus faecalis ?
-
 ?
1.7.1.6 methyl red + NADH + H+
-
 Enterococcus faecalis ATCC 27274 ?
-
 ?
1.7.1.6 methyl red + NADPH + H+
-
 Enterococcus faecalis ?
-
 ?
1.7.1.6 methyl red + NADPH + H+
-
 Enterococcus faecalis ATCC 27274 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 AzoA
-
 Enterococcus faecalis
1.7.1.6 FMN-dependent NAD(P)H azoreductase
-
 Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 FMN dependent on Enterococcus faecalis
1.7.1.6 NADH AzoA shows more than 180fold preference for NADH over NADPH as an electron donor to reduce methyl red Enterococcus faecalis
1.7.1.6 NADPH AzoA shows more than 180fold preference for NADH over NADPH as an electron donor to reduce methyl red Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7.1.6 0.0019
-
 Methyl red in 50 mM potassium phosphate buffer pH 7.0 at 25°C Enterococcus faecalis