Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Luginbuehl, E.; Kunz, S.; Wentzinger, L.; Freimoser, F.; Seebeck, T.
    The exopolyphosphatase TbrPPX1 of Trypanosoma brucei (2011), BMC Microbiol., 11, 4-4.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.6.1.11 additional information the activity of PPX1 is not affected by cAMP, deoxynucleoside triphosphates, ATP, sodium diphosphate, L-arginine, or by long polyanions such as heparin or RNA Trypanosoma brucei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.11 expressed in Escherichia coli BL21(DE3) cells Trypanosoma brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.11 additional information PPX1 is not inhibited by Ro-20-1724, sildenafil, zaprinast, papaverine or etazolate, or the sodium salts of vanadate, fluoride or sulfate Trypanosoma brucei
3.6.1.11 Zn2+ strong inhibitor Trypanosoma brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.11 0.0272
-
 pentasodium triphosphate in 50 mM HEPES, pH 7.8, 0.05 mM EGTA,1 mM MgCl2, at 30°C Trypanosoma brucei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.11 cytoplasm
-
 Trypanosoma brucei 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.11 Mg2+ dependent on Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.11 42800
-
 SDS-PAGE Trypanosoma brucei
3.6.1.11 42870
-
 calculated from amino acid sequence Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.11 Trypanosoma brucei Q7Z032
-
-
3.6.1.11 Trypanosoma brucei 427 Q7Z032
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.11 glutathione-Sepharose resin column chromatography or amylose-resin column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.11 (polyphosphate)n + H2O
-
 Trypanosoma brucei (polyphosphate)n-1 + phosphate
-
 ?
3.6.1.11 (polyphosphate)n + H2O
-
 Trypanosoma brucei 427 (polyphosphate)n-1 + phosphate
-
 ?
3.6.1.11 additional information the PPX1 does not hydrolyze organic triphosphates such as ATP, diphosphate or long-chain polyphosphates. PPX1 does not contain a cyclic-nucleotide specific phosphodiesterase activity Trypanosoma brucei ?
-
 ?
3.6.1.11 additional information the PPX1 does not hydrolyze organic triphosphates such as ATP, diphosphate or long-chain polyphosphates. PPX1 does not contain a cyclic-nucleotide specific phosphodiesterase activity Trypanosoma brucei 427 ?
-
 ?
3.6.1.11 pentasodium triphosphate + H2O
-
 Trypanosoma brucei pentasodium diphosphate + phosphate
-
 ?
3.6.1.11 pentasodium triphosphate + H2O
-
 Trypanosoma brucei 427 pentasodium diphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.11 PPX1
-
 Trypanosoma brucei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.11 8.1
-
 pentasodium triphosphate in 50 mM HEPES, pH 7.8, 0.05 mM EGTA,1 mM MgCl2, at 30°C Trypanosoma brucei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.11 Trypanosoma brucei calculated from amino acid sequence
-
 5.4

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.6.1.11 0.0213
-
 in 50 mM HEPES, pH 7.8, 0.05 mM EGTA,1 mM MgCl2, at 30°C Trypanosoma brucei Zn2+

General Information

EC Number General Information Comment Organism
3.6.1.11 malfunction PPX1 genetic ablation does not produce a dramatic phenotype Trypanosoma brucei