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Literature summary extracted from

  • Chen, H.; Feng, J.; Kweon, O.; Xu, H.; Cerniglia, C.
    Identification and molecular characterization of a novel flavin-free NADPH preferred azoreductase encoded by azoB in Pigmentiphaga kullae K24 (2010), BMC Biochem., 11, 13.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.6 expressed in Escherichia coli BL21-Gold(DE3)pLysS cells Pigmentiphaga kullae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.6 0.001
-
 NADPH pH 6.8, 45°C Pigmentiphaga kullae
1.7.1.6 0.003
-
 Orange I using NADPH as proton donor, at pH 6.8, 45°C Pigmentiphaga kullae
1.7.1.6 0.0086
-
 NADH pH 6.8, 45°C Pigmentiphaga kullae
1.7.1.6 0.17
-
 Orange I using NADH as proton donor, at pH 6.8, 45°C Pigmentiphaga kullae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.6 21300
-
 calculated from amino acid sequence Pigmentiphaga kullae
1.7.1.6 22000
-
 SDS-PAGE and gel filtration Pigmentiphaga kullae

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.6 Pigmentiphaga kullae D5HN83
-
-
1.7.1.6 Pigmentiphaga kullae K24, ATCC BAA-795 D5HN83
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.6 phenyl Sepharose column chromatography and HiPrep SP XL column chromatography Pigmentiphaga kullae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.6 2.9
-
 crude extract, using Orange I as the substrate with NADH as proton donor, pH 6.8, 45°C Pigmentiphaga kullae
1.7.1.6 10.1
-
 purified enzyme, using Orange I as the substrate with NADH as proton donor, pH 6.8, 45°C Pigmentiphaga kullae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.6 additional information Methyl Red, Amaranth, Ponceau BS, Ponceau S, Orange II, Orange G, Megneson II, 1-(4-nitrophenylazo)-2-naphthol, and 4-(4-nitrophenylazo)-resorcinol are not reduced by AzoB Pigmentiphaga kullae ?
-
 ?
1.7.1.6 additional information Methyl Red, Amaranth, Ponceau BS, Ponceau S, Orange II, Orange G, Megneson II, 1-(4-nitrophenylazo)-2-naphthol, and 4-(4-nitrophenylazo)-resorcinol are not reduced by AzoB Pigmentiphaga kullae K24, ATCC BAA-795 ?
-
 ?
1.7.1.6 N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+
-
 Pigmentiphaga kullae 4-(dimethylamino)azobenzene + 2 NADPH + 2 H+
-
 ?
1.7.1.6 N,N-dimethyl-1,4-phenylenediamine + aniline + 2 NADP+
-
 Pigmentiphaga kullae K24, ATCC BAA-795 4-(dimethylamino)azobenzene + 2 NADPH + 2 H+
-
 ?
1.7.1.6 Orange I + NADH both NADH and NADPH can be used as an electron donor for its activity with 4-(4-hydroxy-1-naphthylazo)benzenesulfonic acid (Orange I) as substrate Pigmentiphaga kullae ?
-
 ?
1.7.1.6 Orange I + NADH both NADH and NADPH can be used as an electron donor for its activity with 4-(4-hydroxy-1-naphthylazo)benzenesulfonic acid (Orange I) as substrate Pigmentiphaga kullae K24, ATCC BAA-795 ?
-
 ?
1.7.1.6 Orange I + NADPH both NADH and NADPH can be used as an electron donor for its activity with 4-(4-hydroxy-1-naphthylazo)benzenesulfonic acid (Orange I) as substrate Pigmentiphaga kullae ?
-
 ?
1.7.1.6 Orange I + NADPH both NADH and NADPH can be used as an electron donor for its activity with 4-(4-hydroxy-1-naphthylazo)benzenesulfonic acid (Orange I) as substrate Pigmentiphaga kullae K24, ATCC BAA-795 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.6 monomer 1 * 22000, SDS-PAGE and gel filtration Pigmentiphaga kullae

Synonyms

EC Number Synonyms Comment Organism
1.7.1.6 AzoB
-
 Pigmentiphaga kullae
1.7.1.6 azoreductase
-
 Pigmentiphaga kullae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.6 45
-
 for activity of the enzyme with Orange I Pigmentiphaga kullae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.6 6
-
 for activity of the enzyme with Orange I, in 50 mM Sorensen's phosphate buffer Pigmentiphaga kullae

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.6 additional information AzoB belongs to the flavin-free azoreductase group Pigmentiphaga kullae
1.7.1.6 NADH when NADPH serves as the electron donor, the activity of the enzyme is 63% higher than that when NADH is used Pigmentiphaga kullae
1.7.1.6 NADPH when NADPH serves as the electron donor, the activity of the enzyme is 63% higher than that when NADH is used Pigmentiphaga kullae