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Literature summary extracted from

  • Ullrich, R.; Liers, C.; Schimpke, S.; Hofrichter, M.
    Purification of homogeneous forms of fungal peroxygenase (2009), Biotechnol. J., 4, 1619-1626.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.2.1 extracellular
-
 Cyclocybe aegerita
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.2.1 46000
-
 SDS-PAGE Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.2.1 glycoprotein
-
 Cyclocybe aegerita

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1 ultrafiltration, SP Sepharose column chromatography, and Mono P column chromatography Cyclocybe aegerita

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.2.1 77
-
 major enzyme form P I, after 33fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication Cyclocybe aegerita
1.11.2.1 94
-
 major enzyme form P II, after 40fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication Cyclocybe aegerita
1.11.2.1 103
-
 major enzyme form P III, after 44fold purification, using veratryl alchol as substrate, pH and temperature not specified in the publication Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 naphthalene + H2O2 naphthalene is regioselectively converted into 1-naphthol and 2-naphthol at a ratio of 12:1 Cyclocybe aegerita 1-naphthol + 2-naphthol + H2O
-
 ?
1.11.2.1 toluene + H2O2 all peroxygenase fractions oxygenate toluene at both the side chain and the aromatic ring with a ratio of side chain versus aromatic hydroxylation of about 2:1 in all cases Cyclocybe aegerita benzyl alcohol + benzaldehyde + benzoic acid + 2-cresol + 4-cresol + methylhydroquinone + H2O
-
 ?
1.11.2.1 veratryl alcohol + H2O2 + H+
-
 Cyclocybe aegerita veratraldehyde + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 aromatic peroxygenase
-
 Cyclocybe aegerita

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.2.1 Cyclocybe aegerita the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms
-
 5.2
1.11.2.1 Cyclocybe aegerita the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms
-
 5.6
1.11.2.1 Cyclocybe aegerita the final protein fraction had a molecular mass of 46 kDa but still consists of several incompletely separated proteins with slightly differing isoelectric points (pI 5.2, 5.6, 6.1), probably representing differently glycosylated isoforms
-
 6.1