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Literature summary extracted from

  • Ge, Y.D.; Cao, Z.Y.; Wang, Z.D.; Chen, L.L.; Zhu, Y.M.; Zhu, G.P.
    Identification and biochemical characterization of a thermostable malate dehydrogenase from the mesophile Streptomyces coelicolor A3(2) (2010), Biosci. Biotechnol. Biochem., 74, 2194-2201.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.37 additional information MDH is not affected by DTT or EDTA Streptomyces coelicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.37 gene mdh, sequence comparisons and phylogenetic analysis, overview, overexpression of His6-tagged MDH in Escherichia coli strain Rosetta(DE3), subcloning in Escherichia coli strain DH5alpha Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.37 (S)-malate substrate inhibition, 60% inhibition at 30 mM Streptomyces coelicolor
1.1.1.37 Co2+ 36% inhibition at 2 mM Streptomyces coelicolor
1.1.1.37 DMSO
-
 Streptomyces coelicolor
1.1.1.37 Fe2+ complete inhibition at 2 mM Streptomyces coelicolor
1.1.1.37 additional information MDH is not affected by DTT or EDTA Streptomyces coelicolor
1.1.1.37 oxaloacetate substrate inhibition, 50% inhibition at 6 mM Streptomyces coelicolor
1.1.1.37 Triton X-100
-
 Streptomyces coelicolor
1.1.1.37 Zn2+ 73% inhibition at 2 mM Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.15
-
 NAD+ pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 0.189
-
 oxaloacetate pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 0.494
-
 (S)-malate pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 0.83
-
 NADH pH 8.5, 30°C Streptomyces coelicolor

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 cytosol cytosolic MDH Streptomyces coelicolor 5829
-
1.1.1.37 mitochondrion mitochondrial MDH Streptomyces coelicolor 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.37 additional information enzyme activity is not or poorly affected by 2 mM of Ca2+, Mn2+, Cu2+, Mg2+, Na+, Li+, K+, Rb+, and Ag+ Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 36675
-
 2 * 36675, MALDI-TOF mass spectrometry Streptomyces coelicolor
1.1.1.37 36675
-
 4 * 36675, MALDI-TOF mass spectrometry Streptomyces coelicolor
1.1.1.37 73350
-
 dimeric enzyme, MALDI-TOF mass spectrometry Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Streptomyces coelicolor
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ Streptomyces coelicolor A3(2)
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Streptomyces coelicolor Q9K3J3 gene mdh
-
1.1.1.37 Streptomyces coelicolor A3(2) Q9K3J3 gene mdh
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 recombinant His6-tagged MDH from Escherichia coli strain Rosetta(DE3) by cobalt affinity chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Streptomyces coelicolor oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ the reverse reaction is highly preferred, with a 1050fold higher kcat/Km value Streptomyces coelicolor oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Streptomyces coelicolor A3(2) oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+ the reverse reaction is highly preferred, with a 1050fold higher kcat/Km value Streptomyces coelicolor A3(2) oxaloacetate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer 2 * 36675, MALDI-TOF mass spectrometry Streptomyces coelicolor
1.1.1.37 More structure analysis and comparison, MALDI-TOF mass spectrometry, overview Streptomyces coelicolor
1.1.1.37 tetramer 4 * 36675, MALDI-TOF mass spectrometry Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 (S)-malate dehydrogenase
-
 Streptomyces coelicolor
1.1.1.37 More MDH belongs to the superfamily of 2-ketoacid NAD(P)+-dependent dehydrogenases Streptomyces coelicolor
1.1.1.37 NAD+-dependent MDH
-
 Streptomyces coelicolor
1.1.1.37 NAD-MDH
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 50
-
 reduction and oxidation Streptomyces coelicolor

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.37 40 60 profile, overview Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.37 40
-
 20 min, purified recombinant enzyme, 82% activity remaining Streptomyces coelicolor
1.1.1.37 50
-
 purified recombinant enzyme, 70% activity remaining after 20 min, 71% after 30 min, 60% after 90 min, and 50% after 120 min Streptomyces coelicolor
1.1.1.37 55
-
 20 min, purified recombinant enzyme, 10% activity remaining Streptomyces coelicolor
1.1.1.37 60
-
 20 min, purified recombinant enzyme, complete inactivation Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.37 3.66
-
 NAD+ pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 4.71
-
 (S)-malate pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 542
-
 NADH pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 1870
-
 oxaloacetate pH 8.5, 30°C Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 6.8
-
 50°C, phosphate buffer, recombinant enzyme, reduction and oxidation Streptomyces coelicolor
1.1.1.37 8.5
-
 30°C, Tris-HCl buffer, recombinant enzyme, reduction and oxidation Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.37 5.7 10 profiles in different buffers, overview Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+ dependent on and specific for Streptomyces coelicolor
1.1.1.37 NADH dependent on and specific for Streptomyces coelicolor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.37 5.8
-
 oxaloacetate pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 12.8
-
 (S)-malate pH 8.5, 30°C Streptomyces coelicolor

General Information

EC Number General Information Comment Organism
1.1.1.37 physiological function mitochondrial and cytosolic MDH isozymes are required for maintaining the balance of NAD+ and NADH in mitochondria and cytosol Streptomyces coelicolor

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.37 9.53
-
 (S)-malate pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 24.4
-
 NAD+ pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 6560
-
 NADH pH 8.5, 30°C Streptomyces coelicolor
1.1.1.37 10000
-
 oxaloacetate pH 8.5, 30°C Streptomyces coelicolor