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Literature summary extracted from

  • Hirooka, K.; Fujita, Y.
    Excess production of Bacillus subtilis quercetin 2,3-dioxygenase affects cell viability in the presence of quercetin (2010), Biosci. Biotechnol. Biochem., 74, 1030-1038.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.24 expressed in Escherichia coli BL21(DE3) cells Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.24 Mn2+ required for activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.24 40000
-
 about 40000 Da, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.24 quercetin + O2 Bacillus subtilis
-
 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?
1.13.11.24 quercetin + O2 Bacillus subtilis 168
-
 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.24 Bacillus subtilis
-
-
-
1.13.11.24 Bacillus subtilis 168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.24 partial purification by ammonium sulfate precipitation and DEAE Toyopearl column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.24 0.0012
-
 native enzyme, using quercetin as substrate, at 25°C, pH 7.5 Bacillus subtilis
1.13.11.24 0.0276
-
 recombinant enzyme, using fisetin as substrate, at 25°C, pH 7.5 Bacillus subtilis
1.13.11.24 1.87
-
 recombinant enzyme, using tamarixetin as substrate, at 25°C, pH 7.5 Bacillus subtilis
1.13.11.24 2.27
-
 recombinant enzyme, using quercetin as substrate, at 25°C, pH 7.5 Bacillus subtilis
1.13.11.24 2.49
-
 recombinant enzyme, using galangin as substrate, at 25°C, pH 7.5 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.24 fisetin + O2 1.22% activity compared to quercetin Bacillus subtilis 2-[[(3,4-dihydroxyphenyl)carbonyl]oxy]-4-hydroxybenzoate + CO
-
 ?
1.13.11.24 fisetin + O2 1.22% activity compared to quercetin Bacillus subtilis 168 2-[[(3,4-dihydroxyphenyl)carbonyl]oxy]-4-hydroxybenzoate + CO
-
 ?
1.13.11.24 galangin + O2 110% activity compared to quercetin Bacillus subtilis 2,4-dihydroxy-6-[(phenylcarbonyl)oxy]benzoate + CO
-
 ?
1.13.11.24 galangin + O2 110% activity compared to quercetin Bacillus subtilis 168 2,4-dihydroxy-6-[(phenylcarbonyl)oxy]benzoate + CO
-
 ?
1.13.11.24 quercetin + O2
-
 Bacillus subtilis 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?
1.13.11.24 quercetin + O2 100% activity Bacillus subtilis 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?
1.13.11.24 quercetin + O2
-
 Bacillus subtilis 168 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?
1.13.11.24 quercetin + O2 100% activity Bacillus subtilis 168 2-protocatechoylphloroglucinolcarboxylate + CO
-
 ?
1.13.11.24 tamarixetin + O2 82.4% activity compared to quercetin Bacillus subtilis 2,4-dihydroxy-6-[[(3-hydroxy-4-methoxyphenyl)carbonyl]oxy]benzoate + CO
-
 ?
1.13.11.24 tamarixetin + O2 82.4% activity compared to quercetin Bacillus subtilis 168 2,4-dihydroxy-6-[[(3-hydroxy-4-methoxyphenyl)carbonyl]oxy]benzoate + CO
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.24 QdoI
-
 Bacillus subtilis