EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | an aldehyde + FMNH2 + O2 | Vibrio harveyi | - |
a carboxylate + FMN + H2O + hnu | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.3 | Vibrio harveyi | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | an aldehyde + FMNH2 + O2 | - |
Vibrio harveyi | a carboxylate + FMN + H2O + hnu | - |
? | |
1.14.14.3 | additional information | FMNH2 binds to a mobile loop of 29 amino acids in the luciferase protein, loop modeling of ligand-free and -bound enzyme, conformation and dynamics, overview | Vibrio harveyi | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.14.3 | More | luciferase is composed of two homologous subunits designated alpha and beta, both of which assume the TIM barrel fold. Although the beta-subunit is required for activity, the catalytic site resides exclusively on the alpha-subunit. The most substantial compositional difference between subunits corresponds to a highly conserved stretch of residues between positions 260 and 290 unique to the alpha chains of luminous bacteria. In the luciferase/FMN complex, the asymmetric unit contains two beta/alpha-heterodimers | Vibrio harveyi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.3 | bacterial luciferase | - |
Vibrio harveyi |
1.14.14.3 | luciferase | - |
Vibrio harveyi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.3 | FMNH2 | binds to a mobile loop of 29 amino acids in the luciferase protein, structure and conformation, overview | Vibrio harveyi |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.3 | physiological function | luciferase is the enzyme responsible for light emission in bioluminescent bacteria, it catalyzes the reaction of reduced flavin mononucleotide FMNH2, O2, and an aliphatic aldehyde to yield FMN, the corresponding carboxylic acid, and blue-green light | Vibrio harveyi |