EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | expressed in Escherichia coli BL21(DE3) cells | Plasmodium falciparum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | dTMP | - |
Homo sapiens | |
3.6.1.23 | dTMP | - |
Plasmodium falciparum | |
3.6.1.23 | fluorodeoxy-UMP | - |
Homo sapiens | |
3.6.1.23 | fluorodeoxy-UMP | - |
Plasmodium falciparum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.0011 | - |
dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 0.00173 | - |
2'-deoxy-5-fluoro-UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.00187 | - |
dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.0061 | - |
2'-deoxy-5-fluoro-UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 0.29 | - |
dTTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.438 | - |
beta-L-2'-dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.465 | - |
dCTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.523 | - |
beta-L-2'-dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 0.54 | - |
UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 0.571 | - |
UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.687 | - |
dCTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 0.761 | - |
dTTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 1.13 | - |
dATP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 1.96 | - |
dGTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Homo sapiens | - |
- |
- |
3.6.1.23 | Plasmodium falciparum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.23 | - |
Homo sapiens |
3.6.1.23 | - |
Plasmodium falciparum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | 2'-deoxy-5-fluoro-UTP + H2O | most specific substrate | Homo sapiens | 2'-deoxy-5-fluoro-UMP + diphosphate | - |
? | |
3.6.1.23 | 2'-deoxy-5-fluoro-UTP + H2O | most specific substrate | Plasmodium falciparum | 2'-deoxy-5-fluoro-UMP + diphosphate | - |
? | |
3.6.1.23 | beta-L-2'-dUTP + H2O | - |
Homo sapiens | beta-L-2'-dUMP + diphosphate | - |
? | |
3.6.1.23 | beta-L-2'-dUTP + H2O | - |
Plasmodium falciparum | beta-L-2'-dUMP + diphosphate | - |
? | |
3.6.1.23 | dATP + H2O | - |
Homo sapiens | dAMP + diphosphate | - |
? | |
3.6.1.23 | dATP + H2O | - |
Plasmodium falciparum | dAMP + diphosphate | - |
? | |
3.6.1.23 | dCTP + H2O | - |
Homo sapiens | dCMP + diphosphate | - |
? | |
3.6.1.23 | dCTP + H2O | - |
Plasmodium falciparum | dCMP + diphosphate | - |
? | |
3.6.1.23 | dGTP + H2O | - |
Homo sapiens | dGMP + diphosphate | - |
? | |
3.6.1.23 | dGTP + H2O | - |
Plasmodium falciparum | dGMP + diphosphate | - |
? | |
3.6.1.23 | dTTP + H2O | - |
Homo sapiens | dTMP + diphosphate | - |
? | |
3.6.1.23 | dTTP + H2O | - |
Plasmodium falciparum | dTMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | most specific substrate | Homo sapiens | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | most specific substrate | Plasmodium falciparum | dUMP + diphosphate | - |
? | |
3.6.1.23 | UTP + H2O | - |
Homo sapiens | UMP + diphosphate | - |
? | |
3.6.1.23 | UTP + H2O | - |
Plasmodium falciparum | UMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.23 | deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Homo sapiens |
3.6.1.23 | deoxyuridine 5'-triphosphate nucleotidohydrolase | - |
Plasmodium falciparum |
3.6.1.23 | dUTPase | - |
Homo sapiens |
3.6.1.23 | dUTPase | - |
Plasmodium falciparum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.00013 | - |
dGTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.00265 | - |
dATP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.00525 | - |
beta-L-2'-dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.0061 | - |
beta-L-2'-dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 0.0092 | - |
dTTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.01121 | - |
UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.01278 | - |
UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 0.01971 | - |
dTTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 0.02172 | - |
dCTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 0.1616 | - |
dCTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 110 | - |
2'-deoxy-5-fluoro-UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum | |
3.6.1.23 | 600 | - |
2'-deoxy-5-fluoro-UTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 6600 | - |
dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Homo sapiens | |
3.6.1.23 | 7100 | - |
dUTP | at 25°C in 25 mM MES, 5 mM NaCl, 25 mM MgCl2, 1 mM beta-mercaptoethanol, at pH 7.0 | Plasmodium falciparum |