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Literature summary extracted from

  • Jaisson, S.; Veiga-da-Cunha, M.; Van Schaftingen, E.
    Molecular identification of omega-amidase, the enzyme that is functionally coupled with glutamine transaminases, as the putative tumor suppressor Nit2 (2009), Biochimie, 91, 1066-1071.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.2 mouse nitrilase 2 is expressed as a His-tagged fusion protein in Escherichia coli and in HEK-293T cells Mus musculus
3.5.1.3 expressed as a fusion ptotein with an N-terminal polyHis tag in Escherichia coli and purified to homogeneity on a HisTrap column Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.2 0.003
-
 2-oxo-succinamate pH 7.2, 30°C Mus musculus
3.5.1.2 0.012
-
 5-methoxy-2,5-dioxopentanoic acid pH 7.2, 30°C Mus musculus
3.5.1.2 0.017
-
 2-oxo-succinamate pH 8.5, 30°C Mus musculus
3.5.1.2 0.14
-
 succinamate pH 8.5, 30°C Mus musculus
3.5.1.2 0.195
-
 2-oxo-glutaramate pH 8.5, 30°C Mus musculus
3.5.1.2 0.23
-
 succinamate pH 7.2, 30°C Mus musculus
3.5.1.2 1.27
-
 Glutaramate pH 8.5, 30°C Mus musculus
3.5.1.2 1.48
-
 Glutaramate pH 7.2, 30°C Mus musculus
3.5.1.3 0.003
-
 2-oxosuccinamate pH 7.2, 30°C Mus musculus
3.5.1.3 0.012
-
 gamma-monomethyl-2-oxoglutaramate pH 7.2, 30°C Mus musculus
3.5.1.3 0.017
-
 2-oxosuccinamate pH 8.5, 30°C Mus musculus
3.5.1.3 0.14
-
 succinamate pH 8.5, 30°C Mus musculus
3.5.1.3 0.195
-
 2-Oxoglutaramate pH 8.5, 30°C Mus musculus
3.5.1.3 0.23
-
 succinamate pH 7.2, 30°C Mus musculus
3.5.1.3 1.27
-
 Glutaramate pH 8.5, 30°C Mus musculus
3.5.1.3 1.48
-
 Glutaramate pH 7.2, 30°C Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.2 Mus musculus
-
-
-
3.5.1.3 Mus musculus
-
 bioinformatic approaches are used to exploit bacterial operons to identify a putative omega-amidase by confirming this identification through characterization of the candidate protein
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.2 using Ni-NTA chromatography Mus musculus
3.5.1.3 recombinant protein, purified to homogeneity on a HisTrap column Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.3 liver
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.2 2-oxo-glutaramate + H2O
-
 Mus musculus ?
-
 ?
3.5.1.2 2-oxo-succinamate + H2O
-
 Mus musculus ?
-
 ?
3.5.1.2 5-methoxy-2,5-dioxopentanoic acid + H2O
-
 Mus musculus methanol + 2-oxoglutaric acid
-
 ?
3.5.1.2 glutaramate + H2O
-
 Mus musculus ?
-
 ?
3.5.1.2 succinamate + H2O
-
 Mus musculus ?
-
 ?
3.5.1.3 2-oxoglutaramate + H2O tested at pH 7.2 and pH 8.5 Mus musculus 2-oxoglutarate + NH3
-
 ?
3.5.1.3 2-oxosuccinamate + H2O tested at pH 7.2 and pH 8.5 Mus musculus oxaloacetate + NH3
-
 ?
3.5.1.3 gamma-monomethyl-2-oxoglutaramate + H2O tested at pH 7.2 and pH 8.5 Mus musculus gamma-monomethyl-2-oxoglutarate + NH3
-
 ?
3.5.1.3 glutaramate + H2O tested at pH 7.2 and pH 8.5 Mus musculus glutarate + NH3
-
 ?
3.5.1.3 additional information L-glutamine and L-asparagine are no substrates at pH 7.2 and pH 8.5 Mus musculus ?
-
 ?
3.5.1.3 succinamate + H2O tested at pH 7.2 and pH 8.5 Mus musculus succinate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.2 Nit 2
-
 Mus musculus
3.5.1.2 nitrilase 2
-
 Mus musculus
3.5.1.2 omega-amidase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.2 30
-
 assay at Mus musculus
3.5.1.3 30
-
 assay at Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.2 0.87
-
 2-oxo-succinamate pH 8.5, 30°C Mus musculus
3.5.1.2 1.15
-
 2-oxo-succinamate pH 7.2, 30°C Mus musculus
3.5.1.2 2
-
 succinamate pH 7.2, 30°C Mus musculus
3.5.1.2 2.8
-
 succinamate pH 8.5, 30°C Mus musculus
3.5.1.2 4.1
-
 Glutaramate pH 7.2, 30°C Mus musculus
3.5.1.2 8.8
-
 Glutaramate pH 8.5, 30°C Mus musculus
3.5.1.2 17.5
-
 2-oxo-glutaramate pH 8.5, 30°C Mus musculus
3.5.1.2 134.5
-
 5-methoxy-2,5-dioxopentanoic acid pH 7.2, 30°C Mus musculus
3.5.1.3 0.87
-
 2-oxosuccinamate pH 8.5, 30°C Mus musculus
3.5.1.3 1.15
-
 2-oxosuccinamate pH 7.2, 30°C Mus musculus
3.5.1.3 2
-
 succinamate pH 7.2, 30°C Mus musculus
3.5.1.3 2.8
-
 succinamate pH 8.5, 30°C Mus musculus
3.5.1.3 4.1
-
 Glutaramate pH 7.2, 30°C Mus musculus
3.5.1.3 8.8
-
 Glutaramate pH 8.5, 30°C Mus musculus
3.5.1.3 17.5
-
 2-Oxoglutaramate pH 8.5, 30°C Mus musculus
3.5.1.3 134.5
-
 gamma-monomethyl-2-oxoglutaramate pH 7.2, 30°C Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.2 8.5
-
 assay at Mus musculus
3.5.1.3 7.2
-
 very low activity on 2-oxoglutaramte as substrate compared to assay at pH 8.5, presumably because Nit2 only acts on the linear forms of this substrate. The generation of the linear form from the predominant, cyclic form is pH-dependent, being much fatsre at pH 8.5 than at pH 7.2 Mus musculus
3.5.1.3 8.5
-
 assay at Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.2 2.8
-
 Glutaramate pH 7.2, 30°C Mus musculus
3.5.1.2 6.9
-
 Glutaramate pH 8.5, 30°C Mus musculus
3.5.1.2 8.7
-
 succinamate pH 7.2, 30°C Mus musculus
3.5.1.2 20
-
 succinamate pH 8.5, 30°C Mus musculus
3.5.1.2 51.2
-
 2-oxo-succinamate pH 8.5, 30°C Mus musculus
3.5.1.2 89.7
-
 2-oxo-glutaramate pH 8.5, 30°C Mus musculus
3.5.1.2 385
-
 2-oxo-succinamate pH 7.2, 30°C Mus musculus
3.5.1.2 11200
-
 5-methoxy-2,5-dioxopentanoic acid pH 7.2, 30°C Mus musculus
3.5.1.3 2.8
-
 Glutaramate pH 7.2, 30°C Mus musculus
3.5.1.3 6.9
-
 Glutaramate pH 8.5, 30°C Mus musculus
3.5.1.3 8.7
-
 succinamate pH 7.2, 30°C Mus musculus
3.5.1.3 20
-
 succinamate pH 8.5, 30°C Mus musculus
3.5.1.3 51.2
-
 2-oxosuccinamate pH 8.5, 30°C Mus musculus
3.5.1.3 89.7
-
 2-Oxoglutaramate pH 8.5, 30°C Mus musculus
3.5.1.3 385
-
 2-oxosuccinamate pH 7.2, 30°C Mus musculus
3.5.1.3 11200
-
 gamma-monomethyl-2-oxoglutaramate pH 7.2, 30°C Mus musculus