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Literature summary extracted from

  • Taramino, S.; Teske, B.; Oliaro-Bosso, S.; Bard, M.; Balliano, G.
    Divergent interactions involving the oxidosqualene cyclase and the steroid-3-ketoreductase in the sterol biosynthetic pathway of mammals and yeasts (2010), Biochim. Biophys. Acta, 1801, 1232-1237.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.7 additional information no protective action of steroid-3-ketoreductase towards OSC is necessary Homo sapiens
5.4.99.7 steroid-3-ketoreductase
-
 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.270 additional information overexpression of 17beta-hydroxysteroid dehydrogenase type 7 in ERG27-deleted yeast cells. Recombinant strains grow more slowly than the control yeast ERG27-overexpressing strain on sterol-deficient media, whereas the growth rate is normal on media supplemented with a 3-ketoreductase substrate. Mammalian steroid-3-ketoreductase expressed in yeast shows full enzymatic functioanlity but is unable to support the functionality of yeast oxidosqualene cyclase as ERG27 does Mus musculus
1.1.1.270 additional information overexpression of 17beta-hydroxysteroid dehydrogenase type 7 in ERG27-deleted yeast cells. Recombinant strains grow more slowly than the control yeast ERG27-overexpressing strain on sterol-deficient media, whereas the growth rate is normal on media supplemented with a 3-ketoreductase substrate. Mammalian steroid-3-ketoreductase expressed in yeast shows full enzymatic functioanlity but is unable to support the functionality of yeast oxidosqualene cyclase as ERG27 does Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.7 (S)-squalene-2,3-epoxide Homo sapiens
-
 Lanosterol
-
 ?
5.4.99.7 (S)-squalene-2,3-epoxide Saccharomyces cerevisiae
-
 Lanosterol
-
 ?
5.4.99.7 (S)-squalene-2,3-epoxide Saccharomyces cerevisiae SCY876 and BTY6-5-3
-
 Lanosterol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.270 Homo sapiens P56937
-
-
1.1.1.270 Mus musculus O88736
-
-
5.4.99.7 Homo sapiens
-
-
-
5.4.99.7 Saccharomyces cerevisiae
-
-
-
5.4.99.7 Saccharomyces cerevisiae SCY876 and BTY6-5-3
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.270 NS0 cell
-
 Mus musculus
-
5.4.99.7 NS-0 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.7 (S)-squalene-2,3-epoxide
-
 Homo sapiens Lanosterol
-
 ?
5.4.99.7 (S)-squalene-2,3-epoxide
-
 Saccharomyces cerevisiae Lanosterol
-
 ?
5.4.99.7 (S)-squalene-2,3-epoxide
-
 Saccharomyces cerevisiae SCY876 and BTY6-5-3 Lanosterol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.270 17beta-hydroxysteroid dehydrogenase type 7
-
 Mus musculus
1.1.1.270 17beta-hydroxysteroid dehydrogenase type 7
-
 Homo sapiens
5.4.99.7 Erg7p
-
 Homo sapiens
5.4.99.7 OSC
-
 Homo sapiens
5.4.99.7 OSC
-
 Saccharomyces cerevisiae
5.4.99.7 Oxidosqualene cyclase
-
 Homo sapiens
5.4.99.7 Oxidosqualene cyclase
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
1.1.1.270 physiological function in NS0 cells deficient in 17beta-hydroxysteroid dehydrogenase type 7 activity, oxidosqualene cyclase is fully active. The protective role of the 3-ketoreductase in yeasts with respect to oxidosqualene cyclase activity is not conserved in mammals Mus musculus