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Literature summary extracted from

  • Yang, H.; Wang, Z.; Shen, Y.; Wang, P.; Jia, X.; Zhao, L.; Zhou, P.; Gong, R.; Li, Z.; Yang, Y.; Chen, D.; Murchie, A.I.; Xu, Y.
    Crystal structure of the nosiheptide-resistance methyltransferase of Streptomyces actuosus (2010), Biochemistry, 49, 6440-6450.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.230 crystal structures of the enzyme and the enzyme in complex with S-adenosyl-L-methionine at 2.0 and 2.1 A resolution, respectively Streptomyces actuosus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.230 D36A mutation significantly decreases activity Streptomyces actuosus
2.1.1.230 E220Q mutant has 10% of activity compared to wild-type enzyme Streptomyces actuosus
2.1.1.230 E35A mutation significantly decreases activity Streptomyces actuosus
2.1.1.230 E91A mutation significantly decreases activity Streptomyces actuosus
2.1.1.230 F88A mutation significantly decreases activity Streptomyces actuosus
2.1.1.230 H258A/E259A double mutaion decreases methyltransferase activity to 40% compared to wild-type activity Streptomyces actuosus
2.1.1.230 R135A mutation significantly decreases activity Streptomyces actuosus
2.1.1.230 R165A mutation significantly decreases activity Streptomyces actuosus
2.1.1.230 R92A mutation significantly decreases activity Streptomyces actuosus
2.1.1.230 S219A mutation slightly decreases activity Streptomyces actuosus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.230 Streptomyces actuosus P52391
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.230
-
 Streptomyces actuosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.230 S-adenosyl-L-methionine + adenosine1067 in Escherichia coli 23S rRNA
-
 Streptomyces actuosus S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in Escherichia coli 23S rRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.230 homodimer dimerization is required for methyltransferase activity Streptomyces actuosus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.230 nosiheptide-resistance methyltransferase
-
 Streptomyces actuosus

General Information

EC Number General Information Comment Organism
2.1.1.230 physiological function methylation at adenosine1067 in 23S rRNA is essential for resistance against nosiheptide, a sulfur peptide antibiotic, which is produced by the nosiheptide-producing strain, Streptomyces actuosus Streptomyces actuosus