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Literature summary extracted from

  • Lee, Y.T.; Wilson, R.F.; Rupniewski, I.; Goodin, D.B.
    P450cam visits an open conformation in the absence of substrate (2010), Biochemistry, 49, 3412-3419.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.1 overexpression of P450cam mutant C334A in Escherichia coli strain BL21(DE3) Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.1 camphor-free or camphor-bound P450cam mutant C334A in the absence of substrate and at high and low K+ concentration, protein in 50 mM Tris, pH 7.4, with or without 2-4 mM camphor, mixed with crystallization solution containing 50 mM Tris, pH 7.4, and 12-22% PEG 8000 with and without 200 mM K+, sitting drop vapour diffusion method, 6°C, X-ray diffraction structure determination and analysis at 1.50-1.79 A resolution Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 C334A the mutation of P450cam increases the protein stability compared to the wild-type enzyme Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida P00183
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.1 recombinant P450cam mutant C334A from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration Pseudomonas putida

Subunits

EC Number Subunits Comment Organism
1.14.15.1 More the camphor-free structure is in an open conformation characterized by a water-filled channel created by the retraction of the F and G helices, disorder of the B' helix, and loss of the K+ binding. site. Presence of K+ alone does not alter the open conformation, while camphor alone is sufficient for closure of the channel Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.15.1 P450cam
-
 Pseudomonas putida