Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Noi, K.; Hirai, H.; Hongo, K.; Mizobata, T.; Kawata, Y.
    A potentially versatile nucleotide hydrolysis activity of group II chaperonin monomers from Thermoplasma acidophilum (2009), Biochemistry, 48, 9405-9415.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.1.7 expression in Escherichia coli Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
5.6.1.7 D391A ATP, ADP, and AMP hydrolysis activities decreased significantly compared to wild type, with the exception of the ATP hydrolysis in presence of Co2+ Thermoplasma acidophilum
5.6.1.7 D394A ATP, ADP, and AMP hydrolysis activities decreased significantly compared to wild type Thermoplasma acidophilum
5.6.1.7 D62A ATP, ADP, and AMP hydrolysis activities decreased significantly compared to wild type Thermoplasma acidophilum
5.6.1.7 D67A ATP, ADP, and AMP hydrolysis activities decreased significantly compared to wild type, with the exception of the ATP hydrolysis in presence of Co2+ Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.1.7 EDTA
-
 Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.1.7 Co2+ Ta-cpn beta1 shows ATPase activity in the presence of Co2+. A weak ATPase activity is observed in the presence of Mn2+ or Mg2+ Thermoplasma acidophilum
5.6.1.7 Co2+ Ta-cpn R1 hydrolyzes ATP in the presence of Co2+, Mn2+, or Mg2+. ATPase activity of the monomeric enzyme is the highest in the presence of Co2+ Thermoplasma acidophilum
5.6.1.7 Mg2+ Ta-cpn beta1 shows ATPase activity in the presence of Co2+. A weak ATPase activity is observed in the presence of Mn2+ or Mg2+ Thermoplasma acidophilum
5.6.1.7 Mg2+ Ta-cpn R1 hydrolyzes ATP in the presence of Co2+, Mn2+, or Mg2+ Thermoplasma acidophilum
5.6.1.7 Mn2+ Ta-cpn beta1 shows ATPase activity in the presence of Co2+. A weak ATPase activity is observed in the presence of Mn2+ or Mg2+ Thermoplasma acidophilum
5.6.1.7 Mn2+ Ta-cpn R1 hydrolyzes ATP in the presence of Co2+, Mn2+, or Mg2+ Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
5.6.1.7 Thermoplasma acidophilum P48424 chaperonin alpha-subunit
-
5.6.1.7 Thermoplasma acidophilum P48425 chaperonin beta-subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.1.7
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.1.7 ADP + H2O + a folded polypeptide ADPase activity in presence of Co2+, almost no hydrolysis in presence of Mn2+ or Mg2+, monomeric enzyme form Thermoplasma acidophilum AMP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 ADP + H2O + a folded polypeptide ADPase activity in presence of Co2+, Mn2+ or Mg2+, monomeric enzyme form Thermoplasma acidophilum AMP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 AMP + H2O AMPase activity in presence of Co2+, almost no hydrolysis in presence of Mn2+ or Mg2+, monomeric enzyme form Thermoplasma acidophilum adenosine + phosphate
-
 ?
5.6.1.7 AMP + H2O AMPase activity in presence of Co2+, Mn2+ or Mg2+, monomeric enzyme form Thermoplasma acidophilum adenosine + phosphate
-
 ?
5.6.1.7 ATP + H2O + a folded polypeptide
-
 Thermoplasma acidophilum ADP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 CTP + H2O + a folded polypeptid
-
 Thermoplasma acidophilum CDP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 CTP + H2O + a folded polypeptide
-
 Thermoplasma acidophilum CDP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 GTP + H2O + a folded polypeptide
-
 Thermoplasma acidophilum GDP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 IDP + H2O + a folded polypeptide
-
 Thermoplasma acidophilum IMP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 ITP + H2O + a folded polypeptide
-
 Thermoplasma acidophilum IDP + phosphate + an unfolded polypeptide
-
 ?
5.6.1.7 UTP + H2O + a folded polypeptide
-
 Thermoplasma acidophilum UDP + phosphate + an unfolded polypeptide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.1.7 Ta-cpn alpha1 monomeric enzyme form Thermoplasma acidophilum
5.6.1.7 Ta-cpn beta1 monomeric enzyme form Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.1.7 55
-
-
 Thermoplasma acidophilum