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Literature summary extracted from

  • Zhou, Q.; Shao, W.L.
    Molecular genetic characterization of the thermostable L-lactate dehydrogenase gene (ldhL) of Thermoanaerobacter ethanolicus JW200 and biochemical characterization of the enzyme (2010), Biochemistry (Moscow), 75, 526-530.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.27 D-fructose 1,6-bisphosphate allosteric Thermoanaerobacter ethanolicus
1.1.1.27 Triton X-100 23% activation at 0.1% Thermoanaerobacter ethanolicus
1.1.1.27 Urea 9% activation at 0.1% Thermoanaerobacter ethanolicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.27 gene ldhL, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli Thermoanaerobacter ethanolicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.27 2-mercaptoethanol 10% inhibition at 1 mM Thermoanaerobacter ethanolicus
1.1.1.27 Ag+ complete inhibition at 1 mM Thermoanaerobacter ethanolicus
1.1.1.27 Cu2+ complete inhibition at 1 mM Thermoanaerobacter ethanolicus
1.1.1.27 DTT 19% inhibition at 1 mM Thermoanaerobacter ethanolicus
1.1.1.27 Fe2+ 74% inhibition at 1 mM Thermoanaerobacter ethanolicus
1.1.1.27 Fe3+ complete inhibition at 1 mM Thermoanaerobacter ethanolicus
1.1.1.27 NAD+ 7% inhibition at 0.5 mM Thermoanaerobacter ethanolicus
1.1.1.27 SDS complete inhibition at 0.1% Thermoanaerobacter ethanolicus
1.1.1.27 Zn2+ complete inhibition at 1 mM Thermoanaerobacter ethanolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 additional information
-
 additional information kinetics, the enzyme is allosteric in presence of D-fructose 1,6-bisphosphate, overview Thermoanaerobacter ethanolicus
1.1.1.27 0.18
-
 pyruvate pH 5.8, 60°C, recombinant enzyme Thermoanaerobacter ethanolicus
1.1.1.27 60
-
 L-lactate pH 7.0, 70°C, recombinant enzyme Thermoanaerobacter ethanolicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.27 additional information not or poorly affected by 1 mM of EDTA, ATP, Mg2+, Co2+, Ca2+, Mn2+, and Ni2+ Thermoanaerobacter ethanolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.27 L-lactate + NAD+ Thermoanaerobacter ethanolicus
-
 pyruvate + NADH + H+
-
 r
1.1.1.27 L-lactate + NAD+ Thermoanaerobacter ethanolicus JW 200
-
 pyruvate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Thermoanaerobacter ethanolicus B1A4R6 gene ldhL
-
1.1.1.27 Thermoanaerobacter ethanolicus JW 200 B1A4R6 gene ldhL
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.27 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity Thermoanaerobacter ethanolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 L-lactate + NAD+
-
 Thermoanaerobacter ethanolicus pyruvate + NADH + H+
-
 r
1.1.1.27 L-lactate + NAD+
-
 Thermoanaerobacter ethanolicus JW 200 pyruvate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.27 More TeLdhL contains a GXGXXG motif common to most NAD-linked dehydrogenases close to the N-terminus, and a putative signal peptide with cleavage site between residue 26 and 27, deletion of the putative signal peptide sequence leads to nonexpression of TeLdhL Thermoanaerobacter ethanolicus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.27 L-(+)-lactate dehydrogenase
-
 Thermoanaerobacter ethanolicus
1.1.1.27 L-LDH
-
 Thermoanaerobacter ethanolicus
1.1.1.27 TeLdhL
-
 Thermoanaerobacter ethanolicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.27 60
-
 pyruvate reduction, recombinant enzyme Thermoanaerobacter ethanolicus
1.1.1.27 70
-
 lactate oxidation, recombinant enzyme Thermoanaerobacter ethanolicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.27 70
-
 purified recombinant enzyme, 1-5 h, pH 5.8-8.2, completely stable Thermoanaerobacter ethanolicus
1.1.1.27 80
-
 purified recombinant enzyme, half-life is 1.5 h Thermoanaerobacter ethanolicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.27 5.8
-
 pyruvate reduction, recombinant enzyme Thermoanaerobacter ethanolicus
1.1.1.27 7
-
 lactate oxidation, recombinant enzyme Thermoanaerobacter ethanolicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.27 additional information
-
 incubation of TeLdhL at lower pH results in a rapid inactivation of the enzyme Thermoanaerobacter ethanolicus
1.1.1.27 5.8 8.2 purified recombinant enzyme, 1-5 h, 70°C, completely stable Thermoanaerobacter ethanolicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.27 NAD+
-
 Thermoanaerobacter ethanolicus
1.1.1.27 NADH
-
 Thermoanaerobacter ethanolicus