BRENDA - Enzyme Database

Identification and functional characterization of isocitrate dehydrogenase 1 (IDH1) mutations in thyroid cancer

Murugan, A.K.; Bojdani, E.; Xing, M.; Biochem. Biophys. Res. Commun. 393, 555-559 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
gene idh1, expression of Myc-tagged wild-type and Myc-tagged mutant enzymes in HEK-293T cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
G123R
site-directed mutagenesis, mutation is located in the catalytic domain, the mutant shows reduced activity compared to the wild-type enzyme
Homo sapiens
1.1.1.42
R132H
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Homo sapiens
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Homo sapiens
O75874
IDH1
-
1.1.1.42
Homo sapiens
P48735
IDH2
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
B-CPAP cell
;
Homo sapiens
-
1.1.1.42
C-643 cell
;
Homo sapiens
-
1.1.1.42
FB-1 cell
;
Homo sapiens
-
1.1.1.42
FTC-133 cell
;
Homo sapiens
-
1.1.1.42
HTh-7 cell
;
Homo sapiens
-
1.1.1.42
HTh-74 cell
;
Homo sapiens
-
1.1.1.42
KAT-18 cell
;
Homo sapiens
-
1.1.1.42
OCUT-1 cell
;
Homo sapiens
-
1.1.1.42
OCUT-2 cell
;
Homo sapiens
-
1.1.1.42
SW-1736 cell
;
Homo sapiens
-
1.1.1.42
thyroid cancer cell
;
Homo sapiens
-
1.1.1.42
thyroid cancer cell line
K1 cell; K1 cell; K5 cell; K5 cell
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
711092
Homo sapiens
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
27
-
assay at
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8
-
assay at
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
;
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
gene idh1, expression of Myc-tagged wild-type and Myc-tagged mutant enzymes in HEK-293T cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
G123R
site-directed mutagenesis, mutation is located in the catalytic domain, the mutant shows reduced activity compared to the wild-type enzyme
Homo sapiens
1.1.1.42
R132H
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Homo sapiens
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
B-CPAP cell
-
Homo sapiens
-
1.1.1.42
C-643 cell
-
Homo sapiens
-
1.1.1.42
FB-1 cell
-
Homo sapiens
-
1.1.1.42
FTC-133 cell
-
Homo sapiens
-
1.1.1.42
HTh-7 cell
-
Homo sapiens
-
1.1.1.42
HTh-74 cell
-
Homo sapiens
-
1.1.1.42
KAT-18 cell
-
Homo sapiens
-
1.1.1.42
OCUT-1 cell
-
Homo sapiens
-
1.1.1.42
OCUT-2 cell
-
Homo sapiens
-
1.1.1.42
SW-1736 cell
-
Homo sapiens
-
1.1.1.42
thyroid cancer cell
-
Homo sapiens
-
1.1.1.42
thyroid cancer cell line
K1 cell; K5 cell
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
711092
Homo sapiens
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
27
-
assay at
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8
-
assay at
Homo sapiens