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Literature summary extracted from

  • Muniz, J.R.; Alves, C.A.; de Pieri, C.; Beltramini, L.M.; Selistre-de-Araujo, H.S.; Vettore, A.L.; da Silva, F.R.; Arruda, P.; Garratt, R.C.; Oliva, G.; Souza, D.H.
    Overexpression, purification, biochemical characterization, and molecular modeling of recombinant GDP-mannosyltransferase (GumH) from Xylella fastidiosa (2004), Biochem. Biophys. Res. Commun., 315, 485-492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.252 expressed in fusion with maltose-binding protein in Escherichia coli BL21(DE3) cells and as His-tagged protein in Rosetta(DE3)pLysS cells Xylella fastidiosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.252 45000
-
 1 * 45000, dynamic light scattering Xylella fastidiosa

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.252 Xylella fastidiosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.252 maltose-binding-protein-fused enzyme is partially purified by amylose column chromatography, His-tagged enzyme is purified by Ni-NTA column chromatography Xylella fastidiosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.252 GDP-mannose + cellobiose-diphosphate-polyprenol carrier lipid GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum Xylella fastidiosa GDP + D-mannose-alpha-(1-3)-cellobiose-diphosphate-polyprenol carrier lipid
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.252 monomer 1 * 45000, dynamic light scattering Xylella fastidiosa

Synonyms

EC Number Synonyms Comment Organism
2.4.1.252 GDP-mannosyltransferase
-
 Xylella fastidiosa
2.4.1.252 gumH
-
 Xylella fastidiosa

General Information

EC Number General Information Comment Organism
2.4.1.252 physiological function GumH enzyme is an alpha-1,3-mannosyltransferase responsible for the transfer of mannose from GDP-mannose to the cellobiose-diphosphate-polyprenol carrier lipid during the assembly of the tetrasaccharide repeat unit of the exopolysaccharide fastidian gum Xylella fastidiosa