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Literature summary extracted from

  • Shi, Y.W.; Liu, X.Q.; Shi, P.; Zhang, X.Y.
    Characterization of zinc-binding properties of a novel imidase from Pseudomonas putida YZ-26 (2010), Arch. Biochem. Biophys., 494, 1-6.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.16 C7G/C108G decrease in absorbance at 500 nm by 12.8%. Less than 10% of wild-type activity Pseudomonas putida
3.5.2.16 H151G 120% of wild-type activity Pseudomonas putida
3.5.2.16 H247A decrease in absorbance at 500 nm by 32.4%. Less than 10% of wild-type activity Pseudomonas putida
3.5.2.16 H86A decrease in absorbance at 500 nm by 14.7%. Less than 10% of wild-type activity Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.16 8-hydroxyquinoline 5 mM, 9.4% residual activity Pseudomonas putida
3.5.2.16 EDTA 5 mM, 43.6% residual activity Pseudomonas putida
3.5.2.16 Pyridine-2,4-dicarboxylate 5 mM, 20.8% residual activity Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.16 Cobalt purified imidase, less the addition of any metals, reveals 0.514 mol zinc, 0.102 mol cobalt, and 0.179 mol nickel per subunit Pseudomonas putida
3.5.2.16 Nickel purified imidase, less the addition of any metals, reveals 0.514 mol zinc, 0.102 mol cobalt, and 0.179 mol nickel per subunit Pseudomonas putida
3.5.2.16 Zinc purified imidase, less the addition of any metals, reveals 0.514 mol zinc, 0.102 mol cobalt, and 0.179 mol nickel per subunit. Enzyme purified from cells grown in zinc-supplemented medium contain an approximate stoichiometric amount of the metal of 1.65 mol zinc per subunit. The activity of the apo-imidase depleted of zinc is recovered with the addition of zinc in the lower concentration of 0-20 micorM, whereas the enzymatic activity is decreased in the presence of high concentration of zinc above 100 microM Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.16 Pseudomonas putida
-
-
-
3.5.2.16 Pseudomonas putida YZ-26
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.5.2.16 the activity of the apo-imidase depleted of zinc is recovered with the addition of zinc in the lower concentration of 0-20 micorM, whereas the enzymatic activity is decreased in the presence of high concentration of zinc above 100 microM Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.16 DL-5-hydantoin + H2O
-
 Pseudomonas putida hydantoic acid monoamide
-
 ?
3.5.2.16 DL-5-hydantoin + H2O
-
 Pseudomonas putida YZ-26 hydantoic acid monoamide
-
 ?