BRENDA - Enzyme Database

Peroxisomal localization and function of NADP+-specific isocitrate dehydrogenases in yeast

Lu, Q.; McAlister-Henn, L.; Arch. Biochem. Biophys. 493, 125-134 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.42
expression of His-tagged IDPA in Escherichia coli strain BL21(DE3), expression of IDPA inm Saccharomyces cerevisiae, it behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequenceand occurs in cytosol and peroxisomes, complementation study in Saccharomyces cerevisiae disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, and IDPA lacking the mitochondrial targeting sequence and containing a different PTS1, which results in the same expression level and subcellular orientation, subcellular localization study, overview
Aspergillus nidulans
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
additional information
construction of IDP1, IDP2, and IDP3 disruption mutants, and of double and triple disruption mutants in haploid strain MMY011. Complementation study in disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, which behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequence, PTS1, and occurs in cytosol and peroxisomes, subcellular localization study, overview. Expression of IDPA lacking the mitochondrial targeting sequence and containing a different PTS1 results in the same expression level and subcellular orientation
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Aspergillus nidulans
5829
-
1.1.1.42
cytosol
IDP2 carrying a type I peroxisomal targeting sequence, IDP2+CKL, is only partially localized to peroxisomes, the enzyme is able to function either instead of peroxisomal IDP3 or as cytosolic IDP2
Saccharomyces cerevisiae
5829
-
1.1.1.42
mitochondrion
IDP1
Saccharomyces cerevisiae
5739
-
1.1.1.42
additional information
subcellular localization study of isozymes, overview
Saccharomyces cerevisiae
-
-
1.1.1.42
peroxisome
-
Aspergillus nidulans
5777
-
1.1.1.42
peroxisome
IDP3 contains a canonical type I peroxisomal targeting sequence, a carboxyl-terminal Cys-Lys-Leu tripeptide. IDP3 is normally strictly peroxisomal
Saccharomyces cerevisiae
5777
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
activates
Aspergillus nidulans
1.1.1.42
Mg2+
activates
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Aspergillus nidulans
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae MMY011
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Aspergillus nidulans
-
-
-
1.1.1.42
Saccharomyces cerevisiae
-
-
-
1.1.1.42
Saccharomyces cerevisiae MMY011
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
recombinant His-tagged IDPA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Aspergillus nidulans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
711006
Saccharomyces cerevisiae
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
711006
Aspergillus nidulans
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
711006
Saccharomyces cerevisiae MMY011
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
IDP2
Saccharomyces cerevisiae
1.1.1.42
8
-
-
Aspergillus nidulans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
5.5
9
80% of maximal activity in the pH range of pH 7.0-9.0
Aspergillus nidulans
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
specific for
Aspergillus nidulans
1.1.1.42
NADP+
specific for
Saccharomyces cerevisiae
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.1.42
Saccharomyces cerevisiae
IDP2, isoelectric focusing
-
6.5
1.1.1.42
Aspergillus nidulans
IDPA, isoelectric focusing
-
7.4
1.1.1.42
Saccharomyces cerevisiae
IDP1, isoelectric focusing
-
8.2
1.1.1.42
Saccharomyces cerevisiae
IDP3, isoelectric focusing
-
8.9
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
expression of His-tagged IDPA in Escherichia coli strain BL21(DE3), expression of IDPA inm Saccharomyces cerevisiae, it behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequenceand occurs in cytosol and peroxisomes, complementation study in Saccharomyces cerevisiae disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, and IDPA lacking the mitochondrial targeting sequence and containing a different PTS1, which results in the same expression level and subcellular orientation, subcellular localization study, overview
Aspergillus nidulans
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
specific for
Aspergillus nidulans
1.1.1.42
NADP+
specific for
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.42
additional information
construction of IDP1, IDP2, and IDP3 disruption mutants, and of double and triple disruption mutants in haploid strain MMY011. Complementation study in disruption mutants expressing the full-length IDPA enzyme from Aspergillus nidulans, which behaves similar to the yeast IDP2 harboring a type I peroxisomal targeting sequence, PTS1, and occurs in cytosol and peroxisomes, subcellular localization study, overview. Expression of IDPA lacking the mitochondrial targeting sequence and containing a different PTS1 results in the same expression level and subcellular orientation
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.42
cytosol
-
Aspergillus nidulans
5829
-
1.1.1.42
cytosol
IDP2 carrying a type I peroxisomal targeting sequence, IDP2+CKL, is only partially localized to peroxisomes, the enzyme is able to function either instead of peroxisomal IDP3 or as cytosolic IDP2
Saccharomyces cerevisiae
5829
-
1.1.1.42
mitochondrion
IDP1
Saccharomyces cerevisiae
5739
-
1.1.1.42
additional information
subcellular localization study of isozymes, overview
Saccharomyces cerevisiae
-
-
1.1.1.42
peroxisome
-
Aspergillus nidulans
5777
-
1.1.1.42
peroxisome
IDP3 contains a canonical type I peroxisomal targeting sequence, a carboxyl-terminal Cys-Lys-Leu tripeptide. IDP3 is normally strictly peroxisomal
Saccharomyces cerevisiae
5777
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
activates
Aspergillus nidulans
1.1.1.42
Mg2+
activates
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Aspergillus nidulans
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Saccharomyces cerevisiae MMY011
-
2-oxoglutarate + NADPH + H+ + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
recombinant His-tagged IDPA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Aspergillus nidulans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
711006
Saccharomyces cerevisiae
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
711006
Aspergillus nidulans
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
711006
Saccharomyces cerevisiae MMY011
2-oxoglutarate + NADPH + H+ + CO2
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.5
-
IDP2
Saccharomyces cerevisiae
1.1.1.42
8
-
-
Aspergillus nidulans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
5.5
9
80% of maximal activity in the pH range of pH 7.0-9.0
Aspergillus nidulans
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.1.1.42
Saccharomyces cerevisiae
IDP2, isoelectric focusing
-
6.5
1.1.1.42
Aspergillus nidulans
IDPA, isoelectric focusing
-
7.4
1.1.1.42
Saccharomyces cerevisiae
IDP1, isoelectric focusing
-
8.2
1.1.1.42
Saccharomyces cerevisiae
IDP3, isoelectric focusing
-
8.9
General Information
EC Number
General Information
Commentary
Organism
1.1.1.42
physiological function
IDP3 provides the NADPH required for beta-oxidation of some fatty acids in the peroxisome
Saccharomyces cerevisiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.1.42
physiological function
IDP3 provides the NADPH required for beta-oxidation of some fatty acids in the peroxisome
Saccharomyces cerevisiae