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Literature summary extracted from

  • Kluge, M.G.; Ullrich, R.; Scheibner, K.; Hofrichter, M.
    Spectrophotometric assay for detection of aromatic hydroxylation catalyzed by fungal haloperoxidase-peroxygenase (2007), Appl. Microbiol. Biotechnol., 75, 1473-1478.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 0.037
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) in sodium citrate buffer, pH 4.5, at 25°C Cyclocybe aegerita
1.11.2.1 0.298
-
 2,6-dimethoxyphenol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 0.32
-
 Naphthalene in potassium phosphate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 1.001
-
 benzyl alcohol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 1.313
-
 H2O2 in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 2.367
-
 veratryl alcohol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1 fast protein liquid chromatography Cyclocybe aegerita

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.2.1 74.8
-
 purified enzyme, using veratryl alcohol as substrate, at pH 7.0 and 25°C Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 2 2,6-dimethoxyphenol + 2 H2O2
-
 Cyclocybe aegerita cerulignone + 2 H2O
-
 ?
1.11.2.1 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Cyclocybe aegerita ?
-
 ?
1.11.2.1 benzyl alcohol + H2O2
-
 Cyclocybe aegerita benzaldehyde + H2O
-
 ?
1.11.2.1 naphthalene + H2O2
-
 Cyclocybe aegerita 1-naphthol + H2O in addition to 1-naphthol a smaller amount of naphthalene is converted to 2 naphthol dependent on pH, at pH 7.0-8.0 3% 2-naphthol and 97% 1-naphthol are formed while at pH 3.0 18% 2-naphthol and 82% 1-naphthol are formed. Traces of 1-naphthol are later oxidized to 1,4-naphthoquinone ?
1.11.2.1 veratryl alcohol + H2O2 + H+
-
 Cyclocybe aegerita veratraldehyde + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 Agrocybe aegerita peroxidase P II, main isoform of Agrocybe aegerita peroxidase Cyclocybe aegerita
1.11.2.1 haloperoxidase-peroxygenase
-
 Cyclocybe aegerita

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 85
-
 veratryl alcohol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 108
-
 2,6-dimethoxyphenol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 166
-
 Naphthalene in potassium phosphate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 269
-
 benzyl alcohol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 283
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) in sodium citrate buffer, pH 4.5, at 25°C Cyclocybe aegerita
1.11.2.1 367
-
 H2O2 in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.2.1 5 8 activity related to 1-naphthol formation shows a broad pH optimum between 5.0 and 8.0 Cyclocybe aegerita

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.2.1 3 9 substantial oxygenation of naphthalene still occurs at pH 3.0 and pH 9.0 (60 and 70% of the maximum conversion rate, respectively) Cyclocybe aegerita

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.2.1 heme
-
 Cyclocybe aegerita

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 35.8
-
 veratryl alcohol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 269
-
 benzyl alcohol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 279
-
 H2O2 in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 361
-
 2,6-dimethoxyphenol in sodium citrate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 517
-
 Naphthalene in potassium phosphate buffer, pH 7.0, at 25°C Cyclocybe aegerita
1.11.2.1 7670
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) in sodium citrate buffer, pH 4.5, at 25°C Cyclocybe aegerita