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Literature summary extracted from

  • Lamb, D.C.; Lei, L.; Zhao, B.; Yuan, H.; Jackson, C.J.; Warrilow, A.G.; Skaug, T.; Dyson, P.J.; Dawson, E.S.; Kelly, S.L.; Hachey, D.L.; Waterman, M.R.
    Streptomyces coelicolor A3(2) CYP102 protein, a novel fatty acid hydroxylase encoded as a heme domain without an N-terminal redox partner (2010), Appl. Environ. Microbiol., 76, 1975-1980.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.1 expressed in Escherichia coli BL21(DE3) cells Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.1 Streptomyces coelicolor
-
-
-
1.14.14.1 Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.1 Ni-NTA column chromatography, gel filtration Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.1 arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor 18-hydroxyarachidonic acid + 14,15-epoxyeicosa-5,8,11-trienoic acid + 11,12-epoxyeicosa-5,8,14-trienoic acid + [oxidized NADPH-hemoprotein reductase] + H2O reaction products generated by isoform CYP102B1 ?
1.14.14.1 arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor A3(2) 18-hydroxyarachidonic acid + 14,15-epoxyeicosa-5,8,11-trienoic acid + 11,12-epoxyeicosa-5,8,14-trienoic acid + [oxidized NADPH-hemoprotein reductase] + H2O reaction products generated by isoform CYP102B1 ?
1.14.14.1 arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor 18-hydroxyarachidonic acid + 14,15-epoxyeicosa-5,8,11-trienoic acid + [oxidized NADPH-hemoprotein reductase] + H2O reaction products generated by isoform CYP102A1 ?
1.14.14.1 arachidonic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor A3(2) 18-hydroxyarachidonic acid + 14,15-epoxyeicosa-5,8,11-trienoic acid + [oxidized NADPH-hemoprotein reductase] + H2O reaction products generated by isoform CYP102A1 ?
1.14.14.1 additional information palmitate, stearate, and oleate show negligible binding to isoform CYP102B1, in contrast to CYP102A1 Streptomyces coelicolor ?
-
 ?
1.14.14.1 additional information palmitate, stearate, and oleate show negligible binding to isoform CYP102B1, in contrast to CYP102A1 Streptomyces coelicolor A3(2) ?
-
 ?
1.14.14.1 oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor ?
-
 ?
1.14.14.1 oleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor A3(2) ?
-
 ?
1.14.14.1 palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor ?
-
 ?
1.14.14.1 palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor A3(2) ?
-
 ?
1.14.14.1 stearic acid + [reduced NADPH-hemoprotein reductase] + O2
-
 Streptomyces coelicolor ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.1 CYP102
-
 Streptomyces coelicolor
1.14.14.1 CYP102A1 isoform, binds arachidonate more tightly than isoform CYP102B1 Streptomyces coelicolor
1.14.14.1 CYP102B1 isoform Streptomyces coelicolor
1.14.14.1 fatty acid hydroxylase
-
 Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.1 heme
-
 Streptomyces coelicolor
1.14.14.1 NADPH
-
 Streptomyces coelicolor

General Information

EC Number General Information Comment Organism
1.14.14.1 physiological function isoform CYP102B1 is not required for normal cell growth and secondary metabolite productio Streptomyces coelicolor