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Literature summary extracted from

  • Doublet, B.; Robin, F.; Casin, I.; Fabre, L.; Le Fleche, A.; Bonnet, R.; Weill, F.X.
    Molecular and biochemical characterization of the natural chromosome-encoded class A beta-lactamase from Pseudomonas luteola (2010), Antimicrob. Agents Chemother., 54, 45-51.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expression in Escherichia coli Pseudomonas luteola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.007
-
 piperacillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 0.008
-
 penicillin G pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 0.014
-
 ticarcillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 0.02
-
 amoxicillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 0.021
-
 cephalothin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 0.024
-
 cefuroxime pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 0.035
-
 ceftazidime pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 0.04
-
 aztreonam pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 0.086
-
 cefotaxime pH and temperature not specified in the publication Pseudomonas luteola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 28900
-
 x * 28900, SDS-PAGE Pseudomonas luteola

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Pseudomonas luteola Q670S6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 amoxicillin + H2O
-
 Pseudomonas luteola (2R,4S)-2-[(R)-[[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 aztreonam + H2O
-
 Pseudomonas luteola [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate
-
 ?
3.5.2.6 cefotaxime + H2O
-
 Pseudomonas luteola (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 ceftazidime + H2O
-
 Pseudomonas luteola (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cefuroxime + H2O
-
 Pseudomonas luteola (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalothin + H2O
-
 Pseudomonas luteola (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O
-
 Pseudomonas luteola (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Pseudomonas luteola (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 ticarcillin + H2O
-
 Pseudomonas luteola (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.6 ? x * 28900, SDS-PAGE Pseudomonas luteola

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 blaLUT-1
-
 Pseudomonas luteola
3.5.2.6 LUT-1 beta-lactamase
-
 Pseudomonas luteola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 1.3
-
 ceftazidime pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 1.5
-
 aztreonam pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 5
-
 ticarcillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 8
-
 piperacillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 21
-
 amoxicillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 31
-
 penicillin G pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 286
-
 cefuroxime pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 452
-
 cefotaxime pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 543
-
 cephalothin pH and temperature not specified in the publication Pseudomonas luteola

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Pseudomonas luteola calculated from sequence, isoelectric focusing
-
 6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.2.6 40
-
 ceftazidime pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 40
-
 aztreonam pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 360
-
 ticarcillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 1050
-
 amoxicillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 1140
-
 piperacillin pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 3870
-
 penicillin G pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 5250
-
 cefotaxime pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 11910
-
 cefuroxime pH and temperature not specified in the publication Pseudomonas luteola
3.5.2.6 25860
-
 cephalothin pH and temperature not specified in the publication Pseudomonas luteola