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Literature summary extracted from

  • Piontek, K.; Ullrich, R.; Liers, C.; Diederichs, K.; Plattner, D.A.; Hofrichter, M.
    Crystallization of a 45 kDa peroxygenase/peroxidase from the mushroom Agrocybe aegerita and structure determination by SAD utilizing only the haem iron (2010), Acta Crystallogr. Sect. F, 66, 693-698.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.2.1 hanging drop vapor diffusion method, using ammonium sulfate as the precipitant a total of three suitable crystal forms are obtained at different pH values (PIIpH46 with 2.0 M ammonium sulfate in 100 mM Tris–HCl pH 8.5, PIIpH56 with 2.4 M ammonium sulfate in 100 mM sodium citrate pH 5.6, and PIIpH85 with 2.0 M ammonium sulfate in 200 mM sodium acetate pH 4.6) Cyclocybe aegerita

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.2.1 45000
-
 SDS-PAGE Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Cyclocybe aegerita
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.2.1 glycoprotein highly glycosylated peroxygenase Cyclocybe aegerita

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1 SP Sepharose column chromatography, Mono Q column chromatography, and Mono S column chromatography Cyclocybe aegerita

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 veratryl alcohol + H2O2 + H+
-
 Cyclocybe aegerita veratraldehyde + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 45 kDa peroxygenase/peroxidase
-
 Cyclocybe aegerita
1.11.2.1 APO
-
 Cyclocybe aegerita
1.11.2.1 aromatic peroxygenase
-
 Cyclocybe aegerita
1.11.2.1 PII
-
 Cyclocybe aegerita

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.2.1 heme
-
 Cyclocybe aegerita

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.2.1 Cyclocybe aegerita the enzyme has two pI-values of 5.6 and 5.2, isoelectric focusing
-
 5.2
1.11.2.1 Cyclocybe aegerita the enzyme has two pI-values of 5.6 and 5.2, isoelectric focusing
-
 5.6