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Literature summary extracted from
- Purification, crystallization and preliminary X-ray analysis of apo glyceraldehyde-3-phosphate dehydrogenase 1 (GAP1) from methicillin-resistant Staphylococcus aureus (MRSA252) (2010), Acta Crystallogr. Sect. F, 66, 506-508.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.2.1.12 | expressed in Escherichia coli M15 cells | Staphylococcus aureus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.2.1.12 | hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 8.2, 28% (w/v) PEG 3350, at 25°C | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Staphylococcus aureus | - |
3-phospho-D-glyceroyl phosphate + NADH | - |
? |  |
| 1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | Staphylococcus aureus MRSA252 | - |
3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.12 | Staphylococcus aureus | Q6GIL8 | - |
- |
| 1.2.1.12 | Staphylococcus aureus MRSA252 | Q6GIL8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.12 | Ni-Sepharose column chromatography and Superdex 75 gel filtration | Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Staphylococcus aureus | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
| 1.2.1.12 | D-glyceraldehyde 3-phosphate + phosphate + NAD+ | - |
Staphylococcus aureus MRSA252 | 3-phospho-D-glyceroyl phosphate + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.2.1.12 | homotetramer | x-ray crystallography | Staphylococcus aureus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.1.12 | GAP1 | - |
Staphylococcus aureus |
| 1.2.1.12 | glyceraldehyde-3-phosphate dehydrogenase 1 | - |
Staphylococcus aureus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.12 | NAD+ | - |
Staphylococcus aureus | |
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Release 2023.1 (January 2023)
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