EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.17 | expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Aquifex aeolicus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.17 | free and ATP-bound enzyme, hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 7.6, 29% (w/v) PEG 3350, and 0.75 M NaCl (free enzyme) or 1 ml 0.1 M Tris-HCl pH 8.8, 25% (w/v) PEG 3350, and 0.2 M NaCl (ATP-bound enzyme) | Aquifex aeolicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.17 | 0.054 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
3.6.1.17 | 0.089 | - |
P1,P6-bis(5'-adenosyl) hexaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
3.6.1.17 | 0.183 | - |
P1,P5-bis(5'-adenosyl) pentaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.17 | Aquifex aeolicus | O66548 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.17 | Toyopearl SuperQ-650M column chromatography, Resource Q column chromatography, Bio-Scale CHT2-I column chromatography, and Superdex 75 gel filtration | Aquifex aeolicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.17 | P1,P4-bis(5'-adenosyl) tetraphosphate + H2O | best substrate | Aquifex aeolicus | ATP + AMP | - |
? | |
3.6.1.17 | P1,P5-bis(5'-adenosyl) pentaphosphate + H2O | - |
Aquifex aeolicus | ATP + ADP | - |
? | |
3.6.1.17 | P1,P6-bis(5'-adenosyl) hexaphosphate + H2O | - |
Aquifex aeolicus | ATP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.17 | Ap4A hydrolase | - |
Aquifex aeolicus |
3.6.1.17 | asymmetric Ap4A hydrolase | - |
Aquifex aeolicus |
3.6.1.17 | asymmetric diadenosine tetraphosphate hydrolase | - |
Aquifex aeolicus |
3.6.1.17 | ATP-producing Ap4A hydrolase | - |
Aquifex aeolicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.17 | 0.2 | - |
P1,P6-bis(5'-adenosyl) hexaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
3.6.1.17 | 1.4 | - |
P1,P5-bis(5'-adenosyl) pentaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
3.6.1.17 | 2.5 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.17 | 2.2 | - |
P1,P6-bis(5'-adenosyl) hexaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
3.6.1.17 | 7.6 | - |
P1,P5-bis(5'-adenosyl) pentaphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus | |
3.6.1.17 | 46 | - |
P1,P4-bis(5'-adenosyl) tetraphosphate | in 50 mM Tris-HCl, 100 mM KCl, 5 mM MgCl2, pH 7.6, at 25°C | Aquifex aeolicus |