Literature summary extracted from

Hu, T.; Zhang, Y.; Li, L.; Wang, K.; Chen, S.; Chen, J.; Ding, J.; Jiang, H.; Shen, X.
Two adjacent mutations on the dimer interface of SARS coronavirus 3C-like protease cause different conformational changes in crystal structure (2009), Virology, 388, 324-334.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.22.69	expressed in Escherichia coli BL21(DE3) cells	SARS coronavirus Tor2

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.4.22.69	hanging drop vapor diffusion method, crystals of S139A mutant are grown from the mother liquor containing 0.1 M MES pH 6.0, 10% (w/v) PEG 6000, 1 mM dithiohtreitol, and 5% (v/v) DMSO, crystals of F140A are grown at three pH values in 0.1 M MES pH 6.0/0.1 M MES pH 6.5/0.1 M Tris pH 7.6, with 10% (w/v) PEG 6000, 1 mM dithiothreitol, and 5% (v/v) DMSO	SARS coronavirus Tor2

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.4.22.69	F140A	mutant F140A is a dimer with the most collapsed active pocket discovered so far, well-reflecting the stabilizing role of this residue, the mutant enzyme is completely inactive	SARS coronavirus Tor2
3.4.22.69	S139A	mutant S139A is a monomer that still retains a small fraction of dimer in solution, which may account for its remaining activity	SARS coronavirus Tor2

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.22.69	SARS coronavirus Tor2	P0C6U8	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.22.69	glutathione Sepharose column chromatography and Superdex 75 gel filtration	SARS coronavirus Tor2

Subunits

EC Number	Subunits	Comment	Organism
3.4.22.69	homodimer	only the dimeric enzyme is active	SARS coronavirus Tor2

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.22.69	3C-like protease	-	SARS coronavirus Tor2
3.4.22.69	3cLpro	-	SARS coronavirus Tor2

General Information

EC Number	General Information	Comment	Organism
3.4.22.69	physiological function	3CLpro is vital for SARS-coronavirus replication	SARS coronavirus Tor2

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Release 2023.1 (January 2023)