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Literature summary extracted from

  • Renalier, M.H.; Joseph, N.; Gaspin, C.; Thebault, P.; Mougin, A.
    The Cm56 tRNA modification in archaea is catalyzed either by a specific 2'-O-methylase, or a C/D sRNP (2005), RNA, 11, 1051-1063.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.206 expression in Escherichia coli BL21 Pyrococcus abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.206 22000
-
 x * 22000, SDS-PAGE Pyrococcus abyssi
2.1.1.206 22262
-
 x * 22262, calculated from sequence Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA Pyrococcus abyssi
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.206 Pyrococcus abyssi Q9UYD1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.206
-
 Pyrococcus abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA
-
 Pyrococcus abyssi S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA in vitro, the tRNA ribose 2'-O-methyltransferase aTrm56 specifically catalyses the AdoMet-dependent 2'-O-ribose methylation of cytidine position 56 in tRNA transcripts. In vitro transcribed Escherichia coli tRNALeu2, yeast tRNAPhe, and Pyrococcus abyssi tRNALeu(CAa) tRNAs are methylated with significant initial rates. Mutations of cytosine at position 56 either by uridine or guanine in tRNA abolish methyl incorporation. A tRNA minisubstrate corresponding to the yeast tRNAAsp transcript missing the D stem-loop, is methylated in vitro at a slightly lower efficiency than the wild-type tRNAAsp. A minisubstrate consisting of the T-loop of tRNAAsp closed by a synthetic stem is also tested. This TSL minisubstrate is still methylated in vitro even though the reaction is 4-5fold less efficient than with the wild type substrate. The complete 3D architecture of the tRNA substrate is not required for aTrm56 catalysis. A T stem-loop structure alone is thus a minimal substrate recognized by aTrm56 Pyrococcus abyssi S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.206 ? x * 22000, SDS-PAGE Pyrococcus abyssi
2.1.1.206 ? x * 22262, calculated from sequence Pyrococcus abyssi

Synonyms

EC Number Synonyms Comment Organism
2.1.1.206 aTrm56
-
 Pyrococcus abyssi
2.1.1.206 PAB1040
-
 Pyrococcus abyssi
2.1.1.206 tRNA ribose 2'-O-methyltransferase aTrm56
-
 Pyrococcus abyssi