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Literature summary extracted from

  • Li, Z.; Sau, A.K.
    Structural studies on Helicobacter pylori 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase using electrospray ionization mass spectrometry: a tetrameric complex composed of dimeric dimers (2009), Rapid Commun. Mass Spectrom., 23, 1573-1578.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.55 medicine enzyme absent in mammals, therefore potential target for the development of new antibiotics Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.55 Cd2+ 4 Cd2+ are bound in native tetrameric enzyme, 2 in dimer, 1 in monomer, Cd2+ reconstituted enzyme is less stable than that of Zn2+, Co2+ and Cu2+ enzymes Helicobacter pylori
2.5.1.55 Co2+ monomer and dimer are each bound with 1 metal ion, tetramer with 2 Helicobacter pylori
2.5.1.55 Cu2+ 4 Cu2+ are bound in native tetrameric enzyme, 2 in dimer, 1 in monomer Helicobacter pylori
2.5.1.55 additional information divalent metal ions play an important role in the quaternary structure of the protein Helicobacter pylori
2.5.1.55 Zn2+ 4 Zn2+ are bound in native tetrameric enzyme, 2 in dimer, 1 in monomer Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.55 30400
-
 monomer with 1 Co2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori
2.5.1.55 30440
-
 monomer with 1 Zn2+ or 1 Cu2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori
2.5.1.55 30480
-
 monomer with 1 Cd2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori
2.5.1.55 60690
-
 dimer with 1 Cd2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori
2.5.1.55 60760
-
 dimer with 1 Co2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori
2.5.1.55 60840
-
 dimer with 2 Zn2+ or 2 Cu2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori
2.5.1.55 121500
-
 tetramer with 2 Co2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori
2.5.1.55 121600
-
 tetramer with 4 Zn2+ or 4 Cu2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori
2.5.1.55 121800
-
 tetramer with 4 Cd2+, ESI-MS, 50 mM ammonium acetate solution, pH 7.5 Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O Helicobacter pylori
-
 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.55 Helicobacter pylori
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.55 apoenzyme + 200 microM metal ion salt + 50 mM Tris-HCl buffer, pH 7.5 dialyzed against 50 mM ammonium acetate buffer, pH 7.5, 4°C Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.55 phosphoenolpyruvate + D-arabinose 5-phosphate + H2O
-
 Helicobacter pylori 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.55 tetramer 4 * 30440 (with 2 Co2+), 4 * 30440 (with 4 Zn2+ or Cu2+), 4 * 30480 (with 4 Cd2+), tetramer formed by 2 dimers each, ESI-MS Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.5.1.55 3-deoxy-D-manno-2-octulosonate-8-phosphate synthase
-
 Helicobacter pylori
2.5.1.55 Kdo8P synthase
-
 Helicobacter pylori