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Literature summary extracted from

  • Liu, X.P.; Li, C.P.; Hou, J.L.; Liu, Y.F.; Liang, R.B.; Liu, J.H.
    Expression and characterization of thymine-DNA glycosylase from Aeropyrum pernix (2010), Protein Expr. Purif., 70, 1-6.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.29 expression of the His-tagged enzyme in Escherichia coli strain BL21 (DE3) Aeropyrum pernix

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.2.29 Ca2+ slight inhibition of the enzymic activity Aeropyrum pernix
3.2.2.29 Co2+ complete inhibition at 5 mM Aeropyrum pernix
3.2.2.29 Cu2+ complete inhibition at 5 mM Aeropyrum pernix
3.2.2.29 Mg2+ slight inhibition of the enzymic activity Aeropyrum pernix
3.2.2.29 Mn2+ complete inhibition at 5 mM Aeropyrum pernix
3.2.2.29 additional information additional mismatch located at 3' of T/G have less inhibitory effect on the thymine removal than that located at 5' of T/G. Two additional mismatches located at each side of T/G completely inhibit the excision of thymine Aeropyrum pernix
3.2.2.29 NaCl NaCl completely inhibits the thymine removal at 0.4 M Aeropyrum pernix
3.2.2.29 Ni2+ complete inhibition at 5 mM Aeropyrum pernix
3.2.2.29 Zn2+ complete inhibition at 5 mM Aeropyrum pernix

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.29 Aeropyrum pernix
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.29 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.29 additional information excision of T:G mismatches in oligonucleotide substrates. Aeropyrum pernix TDG also has a relatively weak DNA glycosylase activity on uracil base, with the following descending order: U/C - U/G = U/T = U/U = U/I = U/AP = U/- - U/A. Additional mismatch located at 3' of T/G have less inhibitory effect on the thymine removal than that located at 5' of T/G. Two additional mismatches located at each side of T/G completely inhibit the excision of thymine Aeropyrum pernix ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.29 TDG
-
 Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.29 65 70 recombinant His-tagged enzyme Aeropyrum pernix

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.29 50 85 activity range, profile, recombinant His-tagged enzyme, overview Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.2.29 70
-
 purified recombinant enzyme, 30 min, completely stable Aeropyrum pernix
3.2.2.29 80
-
 purified recombinant enzyme, 30 min, 50% activity remaining Aeropyrum pernix
3.2.2.29 90
-
 purified recombinant enzyme, 5 min, loss of 60% activity, inactive after 30 min Aeropyrum pernix
3.2.2.29 95
-
 purified recombinant enzyme, 5 min, inactivation Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.29 7 8.5 recombinant His-tagged enzyme Aeropyrum pernix

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.29 5 9.5 activity range, profile, recombinant His-tagged enzyme, overview Aeropyrum pernix