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Literature summary extracted from

  • Davies, A.; Kadurin, I.; Alvarez-Laviada, A.; Douglas, L.; Nieto-Rostro, M.; Bauer, C.S.; Pratt, W.S.; Dolphin, A.C.
    The alpha2delta subunits of voltage-gated calcium channels form GPI-anchored proteins, a posttranslational modification essential for function (2010), Proc. Natl. Acad. Sci. USA, 107, 1654-1659.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.13 synthetic construct
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-
-
4.6.1.14 Trypanosoma brucei
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-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.13 additional information PI-PLC does not affect control transmembrane or membrane-associated proteins synthetic construct ?
-
 ?
4.6.1.14 additional information hippocampal detergent-resistant membranes: alpha2beta subunits of voltage-gated calcium channels, alpha2delta-1, alpha2delt-2, and alpha2delt-3, show all properties expected of GPI-anchored proteins, which are substrates for GPI-PLC Trypanosoma brucei ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.13 phosphatidylinositolphospholipase C
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 synthetic construct
4.6.1.13 PI-PLC
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 synthetic construct
4.6.1.14 GPI-phosphatidylinositol-phospholipase C
-
 Trypanosoma brucei
4.6.1.14 GPI-PLC
-
 Trypanosoma brucei

General Information

EC Number General Information Comment Organism
4.6.1.13 physiological function acute incubation of Cos-7 cells for 1 h with PI-PLC significantly reduces the amount of cell-surface expression, both of 5'-nucleotidase by 31.9% and of expressed alpha2delta-2 by 49.4%. PI-PLC treatment reduces calcium current density when alpha2delta subunits are coexpressed. PI-PLC treatment of hippocampal lysates reduces the detergent-resistant membrane association of alpha2delta-1 from 68.3% to 8.3%, and also resultes in recovery of alpha2-1 in the aqueous phase after Triton X-114 separation. Incubation of cultured dorsal root-ganglion neurons for 60 min with PI-PLC substantially reduces cell surface alpha2delta-1 immunofluorescence. It does not reduce that for the transmembrane p75 nerve growth-factor receptor synthetic construct