BRENDA - Enzyme Database

Structure and kinetic investigation of Streptococcus pyogenes family GH38 alpha-mannosidase

Suits, M.D.; Zhu, Y.; Taylor, E.J.; Walton, J.; Zechel, D.L.; Gilbert, H.J.; Davies, G.J.; PLoS ONE 5, e9006 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.24
gene M1 GAS SF370, DNA and amino acid sequence determination and analysis, cloning of the coding sequence for SpGH38 residues 1-901, overexpression as 3C protease cleavable N-terminal His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptococcus pyogenes M1 GAS
3.2.1.114
gene M1 GAS SF370, DNA and amino acid sequence determination and analysis, cloning of the coding sequence for SpGH38 residues 1-901, overexpression as 3C protease cleavable N-terminal His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptococcus pyogenes M1 GAS
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
3.2.1.24
purified recombinent enzyme free or in complex with the inhibitor swainsonine, sitting drop vapour diffusion method, mixing of 12 mg/ml protein in 100 mM Tris, pH 8.5, 1.5 M (NH4)2SO4 and 12% v/v glycerol, with reservoir solution, containing 3% v/v glycerol, 54% v/v Tacsimate, pH 7.0, and 2% v/v PEG 6000, also acting as the cryo-protectant, crystals of the swainsonine complex form are obtained by soaking SpGH38 crystals for ,16 h in mother liquor supplemented with 2 mM swainsonine, X-ray diffraction structure determination and analysis at 1.9 A and 2.6 A resolution, respectively
Streptococcus pyogenes M1 GAS
3.2.1.114
purified recombinent enzyme free or in complex with the inhibitor swainsonine, sitting drop vapour diffusion method, mixing of 12 mg/ml protein in 100 mM Tris, pH 8.5, 1.5 M (NH4)2SO4 and 12% v/v glycerol, with reservoir solution, containing 3% v/v glycerol, 54% v/v Tacsimate, pH 7.0, and 2% v/v PEG 6000, also acting as the cryo-protectant, crystals of the swainsonine complex form are obtained by soaking SpGH38 crystals for ,16 h in mother liquor supplemented with 2 mM swainsonine, X-ray diffraction structure determination and analysis at 1.9 A and 2.6 A resolution, respectively
Streptococcus pyogenes M1 GAS
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.24
swainsonine
binding structure, overview
Streptococcus pyogenes M1 GAS
3.2.1.114
swainsonine
binding structure, overview
Streptococcus pyogenes M1 GAS
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.24
3.6
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.24
27
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
3.6
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
27
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.24
intracellular
SpGH38 may be intracellular, absence of any classical signal peptide
Streptococcus pyogenes M1 GAS
5622
-
3.2.1.114
intracellular
SpGH38 may be intracellular, absence of any classical signal peptide
Streptococcus pyogenes M1 GAS
5622
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.24
Zn2+
involved in catalysis
Streptococcus pyogenes M1 GAS
3.2.1.114
Zn2+
involved in catalysis
Streptococcus pyogenes M1 GAS
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.24
high mannose N-glycan + H2O
Streptococcus pyogenes M1 GAS
-
?
-
-
?
3.2.1.24
additional information
Streptococcus pyogenes M1 GAS
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
?
-
-
-
3.2.1.114
high mannose N-glycan + H2O
Streptococcus pyogenes M1 GAS
-
?
-
-
?
3.2.1.114
additional information
Streptococcus pyogenes M1 GAS
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
?
-
-
-
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.24
Streptococcus pyogenes M1 GAS
Q99YP5
-
-
3.2.1.114
Streptococcus pyogenes M1 GAS
Q99YP5
gene M1 GAS SF370
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.24
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Streptococcus pyogenes M1 GAS
3.2.1.114
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Streptococcus pyogenes M1 GAS
Reaction
EC Number
Reaction
Commentary
Organism
Reaction ID
3.2.1.114
Man5GlcNAc3-[protein] + 2 H2O = Man3GlcNAc3-[protein] + 2 alpha-D-mannopyranose
reaction mechanism via glycosyl-enzyme intermediate, overview
Streptococcus pyogenes M1 GAS
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.24
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
2,4-dinitrophenol + alpha-D-mannopyranose
-
-
-
?
3.2.1.24
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
the enzyme is only active with the alpha-1,3-linked disaccharide
710392
Streptococcus pyogenes M1 GAS
alpha-D-mannopyranose
-
-
-
?
3.2.1.24
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
4-methylumbelliferol + alpha-D-mannopyranose
-
-
-
?
3.2.1.24
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
4-nitrophenol + alpha-D-mannopyranose
-
-
-
?
3.2.1.24
high mannose N-glycan + H2O
-
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
?
3.2.1.24
additional information
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
-
3.2.1.24
additional information
SpGH38 is an alpha-mannosidase with specificity for alpha-1,3 mannosidic linkages, active site structure, overview
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
-
3.2.1.114
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
2,4-dinitrophenol + alpha-D-mannopyranose
-
-
-
?
3.2.1.114
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
the enzyme is only active with the alpha-1,3-linked disaccharide
710392
Streptococcus pyogenes M1 GAS
alpha-D-mannopyranose
-
-
-
?
3.2.1.114
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
4-methylumbelliferol + alpha-D-mannopyranose
-
-
-
?
3.2.1.114
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
4-nitrophenol + alpha-D-mannopyranose
-
-
-
?
3.2.1.114
high mannose N-glycan + H2O
-
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
?
3.2.1.114
additional information
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
-
3.2.1.114
additional information
SpGH38 is an alpha-mannosidase with specificity for alpha-1,3 mannosidic linkages, active site structure, overview
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.24
More
structure comparisons, overview
Streptococcus pyogenes M1 GAS
3.2.1.114
More
structure comparisons, overview
Streptococcus pyogenes M1 GAS
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.24
alpha-1,3 mannosidase
-
Streptococcus pyogenes M1 GAS
3.2.1.24
alpha-mannosidase
-
Streptococcus pyogenes M1 GAS
3.2.1.24
GH38 alpha-mannosidase II
-
Streptococcus pyogenes M1 GAS
3.2.1.24
GH38 enzyme
-
Streptococcus pyogenes M1 GAS
3.2.1.24
More
the enzyme belongs to the family GH38 of alpha-mannosidases
Streptococcus pyogenes M1 GAS
3.2.1.24
SpGH38
-
Streptococcus pyogenes M1 GAS
3.2.1.114
alpha-1,3 mannosidase
-
Streptococcus pyogenes M1 GAS
3.2.1.114
alpha-mannosidase
-
Streptococcus pyogenes M1 GAS
3.2.1.114
GH38 alpha-mannosidase II
-
Streptococcus pyogenes M1 GAS
3.2.1.114
GH38 enzyme
-
Streptococcus pyogenes M1 GAS
3.2.1.114
More
the enzyme belongs to the family GH38 of alpha-mannosidases
Streptococcus pyogenes M1 GAS
3.2.1.114
SpGH38
-
Streptococcus pyogenes M1 GAS
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.24
37
-
assay at
Streptococcus pyogenes M1 GAS
3.2.1.114
37
-
assay at
Streptococcus pyogenes M1 GAS
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.24
0.03
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.24
6.4
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
0.03
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
6.4
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.24
6.8
-
assay at
Streptococcus pyogenes M1 GAS
3.2.1.114
6.8
-
assay at
Streptococcus pyogenes M1 GAS
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.24
0.018
-
swainsonine
pH 6.8, 37°C
Streptococcus pyogenes M1 GAS
3.2.1.114
0.018
-
swainsonine
-
Streptococcus pyogenes M1 GAS
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.24
gene M1 GAS SF370, DNA and amino acid sequence determination and analysis, cloning of the coding sequence for SpGH38 residues 1-901, overexpression as 3C protease cleavable N-terminal His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptococcus pyogenes M1 GAS
3.2.1.114
gene M1 GAS SF370, DNA and amino acid sequence determination and analysis, cloning of the coding sequence for SpGH38 residues 1-901, overexpression as 3C protease cleavable N-terminal His6-tagged enzyme in Escherichia coli strain BL21(DE3)
Streptococcus pyogenes M1 GAS
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
3.2.1.24
purified recombinent enzyme free or in complex with the inhibitor swainsonine, sitting drop vapour diffusion method, mixing of 12 mg/ml protein in 100 mM Tris, pH 8.5, 1.5 M (NH4)2SO4 and 12% v/v glycerol, with reservoir solution, containing 3% v/v glycerol, 54% v/v Tacsimate, pH 7.0, and 2% v/v PEG 6000, also acting as the cryo-protectant, crystals of the swainsonine complex form are obtained by soaking SpGH38 crystals for ,16 h in mother liquor supplemented with 2 mM swainsonine, X-ray diffraction structure determination and analysis at 1.9 A and 2.6 A resolution, respectively
Streptococcus pyogenes M1 GAS
3.2.1.114
purified recombinent enzyme free or in complex with the inhibitor swainsonine, sitting drop vapour diffusion method, mixing of 12 mg/ml protein in 100 mM Tris, pH 8.5, 1.5 M (NH4)2SO4 and 12% v/v glycerol, with reservoir solution, containing 3% v/v glycerol, 54% v/v Tacsimate, pH 7.0, and 2% v/v PEG 6000, also acting as the cryo-protectant, crystals of the swainsonine complex form are obtained by soaking SpGH38 crystals for ,16 h in mother liquor supplemented with 2 mM swainsonine, X-ray diffraction structure determination and analysis at 1.9 A and 2.6 A resolution, respectively
Streptococcus pyogenes M1 GAS
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.24
swainsonine
binding structure, overview
Streptococcus pyogenes M1 GAS
3.2.1.114
swainsonine
binding structure, overview
Streptococcus pyogenes M1 GAS
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.24
0.018
-
swainsonine
pH 6.8, 37°C
Streptococcus pyogenes M1 GAS
3.2.1.114
0.018
-
swainsonine
-
Streptococcus pyogenes M1 GAS
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.24
3.6
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.24
27
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
3.6
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
27
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.24
intracellular
SpGH38 may be intracellular, absence of any classical signal peptide
Streptococcus pyogenes M1 GAS
5622
-
3.2.1.114
intracellular
SpGH38 may be intracellular, absence of any classical signal peptide
Streptococcus pyogenes M1 GAS
5622
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.24
Zn2+
involved in catalysis
Streptococcus pyogenes M1 GAS
3.2.1.114
Zn2+
involved in catalysis
Streptococcus pyogenes M1 GAS
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.2.1.24
high mannose N-glycan + H2O
Streptococcus pyogenes M1 GAS
-
?
-
-
?
3.2.1.24
additional information
Streptococcus pyogenes M1 GAS
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
?
-
-
-
3.2.1.114
high mannose N-glycan + H2O
Streptococcus pyogenes M1 GAS
-
?
-
-
?
3.2.1.114
additional information
Streptococcus pyogenes M1 GAS
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.24
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Streptococcus pyogenes M1 GAS
3.2.1.114
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
Streptococcus pyogenes M1 GAS
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.24
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
2,4-dinitrophenol + alpha-D-mannopyranose
-
-
-
?
3.2.1.24
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
the enzyme is only active with the alpha-1,3-linked disaccharide
710392
Streptococcus pyogenes M1 GAS
alpha-D-mannopyranose
-
-
-
?
3.2.1.24
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
4-methylumbelliferol + alpha-D-mannopyranose
-
-
-
?
3.2.1.24
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
4-nitrophenol + alpha-D-mannopyranose
-
-
-
?
3.2.1.24
high mannose N-glycan + H2O
-
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
?
3.2.1.24
additional information
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
-
3.2.1.24
additional information
SpGH38 is an alpha-mannosidase with specificity for alpha-1,3 mannosidic linkages, active site structure, overview
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
-
3.2.1.114
2,4-dinitrophenyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
2,4-dinitrophenol + alpha-D-mannopyranose
-
-
-
?
3.2.1.114
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose + H2O
the enzyme is only active with the alpha-1,3-linked disaccharide
710392
Streptococcus pyogenes M1 GAS
alpha-D-mannopyranose
-
-
-
?
3.2.1.114
4-methylumbelliferyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
4-methylumbelliferol + alpha-D-mannopyranose
-
-
-
?
3.2.1.114
4-nitrophenyl alpha-D-mannopyranoside + H2O
-
710392
Streptococcus pyogenes M1 GAS
4-nitrophenol + alpha-D-mannopyranose
-
-
-
?
3.2.1.114
high mannose N-glycan + H2O
-
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
?
3.2.1.114
additional information
higher eukaryotic GH38 alpha-mannosides play a key role in the modification and diversification of hybrid N-glycans, processes with strong cellular links to cancer and autoimmune disease
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
-
3.2.1.114
additional information
SpGH38 is an alpha-mannosidase with specificity for alpha-1,3 mannosidic linkages, active site structure, overview
710392
Streptococcus pyogenes M1 GAS
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.24
More
structure comparisons, overview
Streptococcus pyogenes M1 GAS
3.2.1.114
More
structure comparisons, overview
Streptococcus pyogenes M1 GAS
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.24
37
-
assay at
Streptococcus pyogenes M1 GAS
3.2.1.114
37
-
assay at
Streptococcus pyogenes M1 GAS
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.2.1.24
0.03
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.24
6.4
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
0.03
-
4-methylumbelliferyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
6.4
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.24
6.8
-
assay at
Streptococcus pyogenes M1 GAS
3.2.1.114
6.8
-
assay at
Streptococcus pyogenes M1 GAS
General Information
EC Number
General Information
Commentary
Organism
3.2.1.24
physiological function
the enzyme is perhaps functioning in the subsequent degradation of extracellular host glycans following their initial digestion by secreted glycosidases
Streptococcus pyogenes M1 GAS
3.2.1.114
physiological function
the enzyme is perhaps functioning in the subsequent degradation of extracellular host glycans following their initial digestion by secreted glycosidases
Streptococcus pyogenes
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.2.1.24
physiological function
the enzyme is perhaps functioning in the subsequent degradation of extracellular host glycans following their initial digestion by secreted glycosidases
Streptococcus pyogenes M1 GAS
3.2.1.114
physiological function
the enzyme is perhaps functioning in the subsequent degradation of extracellular host glycans following their initial digestion by secreted glycosidases
Streptococcus pyogenes
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.2.1.24
0.01
-
2,4-dinitrophenyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.24
0.24
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
0.01
-
2,4-dinitrophenyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes
3.2.1.114
0.24
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
3.2.1.24
0.01
-
2,4-dinitrophenyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.24
0.24
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes M1 GAS
3.2.1.114
0.01
-
2,4-dinitrophenyl alpha-D-mannopyranoside
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes
3.2.1.114
0.24
-
3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
pH 6.8, 37°C, recombinant enzyme
Streptococcus pyogenes