EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.22.1 | nuc-1, crn-7 or crn-6 coding sequence without a stop codon amplified and inserted into the pPD95.79 vector. Transcriptional reporter constructs containing the promoter of the DNase II gene fused to GFP with four tandem copies of the nuclear localization signal (NLS) injected into wild-type animals (embryos, larvae and adults) | Caenorhabditis elegans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.22.1 | Caenorhabditis elegans | - |
N2 Bristol strain | - |
3.1.22.1 | Caenorhabditis elegans N2 Bristol | - |
N2 Bristol strain | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.22.1 | body wall muscle | in Pnuc-1(4xNLS)::GFP transgenic larvae and adults, and in Pcrn-7(4xNLS)::GFP larvae | Caenorhabditis elegans | - |
3.1.22.1 | head | in Pnuc-1(4xNLS)::GFP transgenic embryos, in the head region of comma and 1.5fold stage embryos and 3fold or 4fold stage embryos, and in Pnuc-1(4xNLS)::GFP transgenic larvae and adults | Caenorhabditis elegans | - |
3.1.22.1 | head muscle | in Pcrn-7(4xNLS)::GFP larvae | Caenorhabditis elegans | - |
3.1.22.1 | intestine | in posterior intestinal cells of Pnuc-1(4xNLS)::GFP transgenic larvae and adults. In anterior intestinal cells of 3fold or 4fold stage Pnuc-1(4xNLS)::GFP transgenic embryos and in Pnuc-1(4xNLS)::GFP transgenic larvae and adults. In intestinal cells of Pcrn-6(4xNLS)::GFP transgenic embryos, larvae or adults | Caenorhabditis elegans | - |
3.1.22.1 | additional information | is present in intestinal precursor cells of Pcrn-6(4xNLS)::GFP transgenic embryos. Is absent from Pcrn-7(4xNLS)::GFP transgenic embryos. Except for a few anterior intestinal cells in 4fold stage embryos, the embryonic expression patterns of the three DNase II genes do not seem to overlap | Caenorhabditis elegans | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.22.1 | DNA + H2O | - |
Caenorhabditis elegans | ? | - |
? | |
3.1.22.1 | DNA + H2O | - |
Caenorhabditis elegans N2 Bristol | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.22.1 | DNase II | - |
Caenorhabditis elegans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.22.1 | malfunction | loss of all three DNase II genes (nuc-1, crn-6 or crn-7) does not affect the activation or progression of cell death. Removal or disruption of NUC-1 secretion signal eliminates NUC-1's ability to mediate DNA degradation across its expression border | Caenorhabditis elegans |
3.1.22.1 | physiological function | three DNase II genes: nuc-1 plays a major role, crn-6 plays an auxiliary role, and crn-7 plays a negligible role in resolving 3' OH DNA breaks generated in apoptotic cells. NUC-1 constitutes the major DNase II activity and CRN-6 provides the minor DNase II activity in the organism. Nuc-1, crn-6 and crn-7 affect apoptotic DNA degradation but do not affect the activation or the kinetics of apoptosis. The crn-6 gene but not crn-7 can partially substitute for nuc-1 in mediating apoptotic DNA degradation and both fail to replace nuc-1 in degrading bacterial DNA in intestine. Despite of their restricted and largely non-overlapping expression patterns, both CRN-6 and NUC-1 can mediate apoptotic DNA degradation in many cells, suggesting that they are likely secreted nucleases that are retaken up by other cells to exert DNA degradation functions. NUC-1, but not CRN-6 or CRN-7, mediates degradation of chromosome DNA in ventral cord apoptotic cells and bacterial DNA in intestine. Neither CRN-6 nor CRN-7 can substitute for NUC-1 in digesting bacterial DNA in intestine. Nuc-1 can still promote apoptotic DNA degradation in both embryos and larvae when its expression is restricted to intestine cells. NUC-1 expressed in the head can mediate apoptotic DNA degradation in the posterior ventral cord | Caenorhabditis elegans |