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Literature summary extracted from

  • Roy Choudhury, S.; Roy, S.; Sengupta, D.N.
    Characterization of cultivar differences in beta-1,3 glucanase gene expression, glucanase activity and fruit pulp softening rates during fruit ripening in three naturally occurring banana cultivars (2009), Plant Cell Rep., 28, 1641-1653.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 cloning from cultivars Rasthali, Kanthali, and Monthan, DNA and amino acid sequence determination and analysis, expression of His-tagged enzymes in Escherichia coli strain M15 Musa x paradisiaca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.39 37000
-
 x * 37000, recombinant His-tagged enzyme, SDS-PAGE Musa x paradisiaca

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Musa x paradisiaca
-
 different cultivars Rasthali, Kanthali, and Monthan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.39 recombinant His-tagged enzymes from Escherichia coli strain M15 by nickel affinity chromatography Musa x paradisiaca

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.39 fruit cultivar differences in beta-1,3 glucanase gene expression, glucanase activity and fruit pulp softening rates during fruit ripening in three naturally occurring banana cultivars. Low enzyme expressiong during fruit ripening Musa x paradisiaca
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.39 0.0025 0.015 activites of purified recombinant enzymes from different cultivars, overview Musa x paradisiaca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 beta-glucan + H2O from cell wall polysaccharide extracts of fruits or from barley, activites and substrate specificities of enzymes from different cultivars, overview Musa x paradisiaca ?
-
 ?
3.2.1.39 laminarin + H2O
-
 Musa x paradisiaca ?
-
 ?
3.2.1.39 pachyman + H2O
-
 Musa x paradisiaca ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 37000, recombinant His-tagged enzyme, SDS-PAGE Musa x paradisiaca

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 beta-1,3 glucanase
-
 Musa x paradisiaca

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 50
-
 assay at Musa x paradisiaca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.39 50 65 purified recombinant enzyme, 30 min, stable Musa x paradisiaca
3.2.1.39 70
-
 purified recombinant enzyme, 10 min, partly loss of activity Musa x paradisiaca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 4.8
-
 assay at Musa x paradisiaca

Expression

EC Number Organism Comment Expression
3.2.1.39 Musa x paradisiaca ethylene treatment differentially stimulates beta-1,3 glucanase expression in the three banana cultivars, overview. Noinduction incv. Monthan, slight induction in cv. Kanthali, promninent induction in cv. Rasthali additional information