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Literature summary extracted from

  • Quiroga, E.N.; Sgariglia, M.A.; Molina, C.F.; Sampietro, D.A.; Soberon, J.R.; Vattuone, M.A.
    Purification and characterization of an exo-polygalacturonase from Pycnoporus sanguineus (2009), Mycol. Res., 113, 1404-1410.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 additional information
-
 additional information kinetics Trametes sanguinea
3.2.1.82 0.55
-
 citric pectin pH 6.8, 37°C Trametes sanguinea
3.2.1.82 0.55
-
 poly(1,4-alpha-D-galactosiduronate) pH 6.8, 37°C Trametes sanguinea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular
-
 Trametes sanguinea
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 42000
-
 1 * 42000, PGase I, SDS-PAGE and PAGE Trametes sanguinea
3.2.1.82 42000
-
 1 * 42000, SDS-PAGE Trametes sanguinea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.67 citric pectin + H2O Trametes sanguinea
-
 galacturonic acid + ?
-
 ?
3.2.1.67 additional information Trametes sanguinea PGase I is an exocellular enzyme releasing galacturonic acid ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O Trametes sanguinea
-
 galacturonic acid + ?
-
 ?
3.2.1.82 citric pectin + H2O Trametes sanguinea PGase I is an exocellular enzyme releasing galacturonic acid as its principal hydrolysis product, it hydrolyzes digalacturonate, and it shows a higher affinity towards poly(1,4-alpha-D-galactosiduronate) than citric pectin ?
-
 ?
3.2.1.82 poly(1,4-alpha-D-galactosiduronate) + H2O Trametes sanguinea i.e. PGA, PGase I is an exocellular enzyme releasing galacturonic acid as its principal hydrolysis product, it hydrolyzes digalacturonate, and it shows a higher affinity towards PGA than citric pectin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Trametes sanguinea
-
-
-
3.2.1.82 Trametes sanguinea
-
 grown on citrus fruit pectin
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.67 glycoprotein the enzyme is N-glycosylated Trametes sanguinea
3.2.1.82 glycoprotein PGase I is an N-glycosidated monomer Trametes sanguinea

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 soluble native PGase I 200fold by ammonium sulfate fractionation and gel filtration to homogeneity Trametes sanguinea
3.2.1.82 native enzyme 200fold by ammonium sulfate fractionation, dialysis, and gel filtration Trametes sanguinea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.67 mycelium
-
 Trametes sanguinea
-
3.2.1.82 mycelium
-
 Trametes sanguinea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.82 500
-
 purified native enzyme Trametes sanguinea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 citric pectin + H2O
-
 Trametes sanguinea galacturonic acid + ?
-
 ?
3.2.1.67 digalacturonate + H2O
-
 Trametes sanguinea 2 galacturonate
-
 ?
3.2.1.67 additional information PGase I is an exocellular enzyme releasing galacturonic acid Trametes sanguinea ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O
-
 Trametes sanguinea galacturonic acid + ?
-
 ?
3.2.1.67 polygalacturonic acid + H2O preferred substrate Trametes sanguinea galacturonic acid + ?
-
 ?
3.2.1.82 citric pectin + H2O PGase I is an exocellular enzyme releasing galacturonic acid as its principal hydrolysis product, it hydrolyzes digalacturonate, and it shows a higher affinity towards poly(1,4-alpha-D-galactosiduronate) than citric pectin Trametes sanguinea ?
-
 ?
3.2.1.82 additional information reaction product determination and analysis, overview Trametes sanguinea ?
-
 ?
3.2.1.82 poly(1,4-alpha-D-galactosiduronate) + H2O i.e. PGA, PGase I is an exocellular enzyme releasing galacturonic acid as its principal hydrolysis product, it hydrolyzes digalacturonate, and it shows a higher affinity towards PGA than citric pectin Trametes sanguinea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 monomer 1 * 42000, PGase I, SDS-PAGE and PAGE Trametes sanguinea
3.2.1.82 monomer 1 * 42000, SDS-PAGE Trametes sanguinea

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exo-PGase
-
 Trametes sanguinea
3.2.1.67 exo-polygalacturonase
-
 Trametes sanguinea
3.2.1.67 PGase I
-
 Trametes sanguinea
3.2.1.82 exo-polygalacturonase
-
 Trametes sanguinea
3.2.1.82 PGase I
-
 Trametes sanguinea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 50 60 PGase I Trametes sanguinea
3.2.1.82 50 60
-
 Trametes sanguinea

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.67 20 70 PGase I Trametes sanguinea
3.2.1.82 20 80 activity range Trametes sanguinea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 20 60 at pH 4.8 the activity of the purified PGase I increases from 20 to 60°C, decreasing rapidly beyond Trametes sanguinea
3.2.1.67 70
-
 purified PGase I, 30 min, loss of 57% activity Trametes sanguinea
3.2.1.67 80
-
 purified PGase I, 30 min, loss of 90% activity Trametes sanguinea
3.2.1.82 60
-
 purified native enzyme, stable up to Trametes sanguinea
3.2.1.82 70
-
 purified native enzyme, loss of 57% activity after 30 min, pH 4.8 Trametes sanguinea
3.2.1.82 80
-
 purified native enzyme, loss of 90% activity after 30 min, pH 4.8 Trametes sanguinea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 4.8
-
 PGase I Trametes sanguinea
3.2.1.82 4.8
-
-
 Trametes sanguinea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.67 2.8 10 PGase I Trametes sanguinea
3.2.1.82 3 8 activity range, profile, overview Trametes sanguinea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 3.8 8 purified PGase I, stable Trametes sanguinea
3.2.1.82 3.8 8 purified native enzyme, stable Trametes sanguinea

Expression

EC Number Organism Comment Expression
3.2.1.67 Trametes sanguinea growth on citrus fruit pectin induces the enzyme up